Liquid chromatography is a technique that is gaining increasing interest and application, for example in the pharmaceutical industry. At the same time as the increase in the number of analyses performed, the amount of organic waste produced while working with high-performance liquid chromatography (HPLC) apparatus is growing. Therefore, new methods and materials are being searched for to achieve the so-called "green" chromatography. In the following review we describe one of them, specifically the replacement of harmful organic solvents such as acetonitrile, methanol or isopropanol, with pure water used as the sole component of the mobile phase. In order to achieve a single component mobile phase, different methods or materials are used: the use of elevated temperature, the selection of new stationary phases, the utilization of changes in the properties of the stationary phase while using a highly polar eluent.

液相色谱法是一种在例如制药工业中越来越受到关注和应用的技术。随着分析数量的增加，使用高效液相色谱（HPLC）仪器工作时产生的有机废物量也在增加。因此，正在寻找新的方法和材料以实现所谓的“绿色”色谱法。在下面的综述中，我们将介绍其中一种，特别是将纯水用作流动相的唯一组分，以代替有害的有机溶剂，例如乙腈，甲醇或异丙醇。为了获得单一组分的流动相，使用了不同的方法或材料：使用高温，选择新的固定相，