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Why do DIETers like drinking: Metagenomic analysis for methane and energy metabolism during anaerobic digestion with ethanol.
Water Research ( IF 11.4 ) Pub Date : 2019-12-23 , DOI: 10.1016/j.watres.2019.115425 Zhiqiang Zhao 1 , Jianfeng Wang 1 , Yang Li 2 , Tingting Zhu 3 , Qilin Yu 1 , Tingting Wang 1 , Song Liang 1 , Yaobin Zhang 1
Water Research ( IF 11.4 ) Pub Date : 2019-12-23 , DOI: 10.1016/j.watres.2019.115425 Zhiqiang Zhao 1 , Jianfeng Wang 1 , Yang Li 2 , Tingting Zhu 3 , Qilin Yu 1 , Tingting Wang 1 , Song Liang 1 , Yaobin Zhang 1
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Stimulating Methanothrix-dominant communities with ethanol is recently considered as a promising strategy of improving the efficiency and stability of anaerobic digestion (AD), while the effects on methanogenic pathway and energy metabolism linked to the establishment of direct interspecies electron transfer (DIET) were not investigated yet. The results showed that, Methanothrix species were the dominant and metabolically active methanogens in the methanogenic sludge fed with the ethanol-type fermentation products, and the abundance of genes that encoded the key enzymes involved in the reduction of carbon dioxide was significantly higher than that fed with the other products, such as propionate and butyrate. Conversely, the abundance of genes that encoded the key enzymes involved in acetate decarboxylation among all the methanogenic sludge were nearly same. In the presence of ethanol, the abundance of gene for pilA significantly increased. The gene for pliA was primarily derived from Sphaerochaeta, Sedimentibacter and Pseudomonas species that were specially abundant and metabolically active. Further analysis showed that, the abundance of genes that encoded V/A-type ATPase in the methanogenic digesters fed with the ethanol-type fermentation products was 1.3-1.5 folds higher than that fed with the other products. As a result, the concentration of total ATP in the cells was increased by 1.8-2.3 folds. These results, and the fact that DIET is the only electron donor to support the reduction of carbon dioxide in Methanothrix species for the first time revealed the mechanisms involved in the establishment of DIET-based methanogenic metabolism with ethanol.
中文翻译：
DIETers为什么喜欢喝酒：用乙醇厌氧消化过程中甲烷和能量代谢的元基因组分析。
最近，以乙醇刺激以甲烷甲烷为主的群落被认为是提高厌氧消化（AD）的效率和稳定性的一种有前途的策略，而与建立直接种间电子转移（DIET）相关的对甲烷生成途径和能量代谢的影响却没有。尚未调查。结果表明，在以乙醇型发酵产物为原料的产甲烷污泥中，甲烷菌属物种是占主导地位且具有代谢活性的产甲烷菌，并且编码与减少二氧化碳有关的关键酶的编码基因的丰度明显高于饲喂甲烷的微生物。与其他产品，例如丙酸酯和丁酸酯。反过来，在所有产甲烷污泥中，编码参与乙酸盐脱羧的关键酶的基因的丰度几乎相同。在乙醇存在下，pilA基因的丰度大大增加。pliA的基因主要来自特别丰富且具有代谢活性的Sphaerochaeta，Sedimentibacter和Pseudomonas物种。进一步的分析表明，饲喂乙醇型发酵产物的产甲烷消化器中编码V / A型ATPase的基因的丰度比饲喂其他发酵产物的产甲烷菌高1.3-1.5倍。结果，细胞中总ATP的浓度增加了1.8-2.3倍。这些结果，
更新日期：2019-12-23
中文翻译：
DIETers为什么喜欢喝酒：用乙醇厌氧消化过程中甲烷和能量代谢的元基因组分析。
最近，以乙醇刺激以甲烷甲烷为主的群落被认为是提高厌氧消化（AD）的效率和稳定性的一种有前途的策略，而与建立直接种间电子转移（DIET）相关的对甲烷生成途径和能量代谢的影响却没有。尚未调查。结果表明，在以乙醇型发酵产物为原料的产甲烷污泥中，甲烷菌属物种是占主导地位且具有代谢活性的产甲烷菌，并且编码与减少二氧化碳有关的关键酶的编码基因的丰度明显高于饲喂甲烷的微生物。与其他产品，例如丙酸酯和丁酸酯。反过来，在所有产甲烷污泥中，编码参与乙酸盐脱羧的关键酶的基因的丰度几乎相同。在乙醇存在下，pilA基因的丰度大大增加。pliA的基因主要来自特别丰富且具有代谢活性的Sphaerochaeta，Sedimentibacter和Pseudomonas物种。进一步的分析表明，饲喂乙醇型发酵产物的产甲烷消化器中编码V / A型ATPase的基因的丰度比饲喂其他发酵产物的产甲烷菌高1.3-1.5倍。结果，细胞中总ATP的浓度增加了1.8-2.3倍。这些结果，
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