Changes in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women.

中文翻译：

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使用过滤器辅助的样品制备方法，改进了对巯基氧化还原蛋白质组的综合分析。

蛋白Cys残基氧化态的变化参与细胞信号传导，并在多种病理生理状态中发挥关键作用。我们之前已经开发了GELSILOX，这是一种凝胶内方法，可以对Cys位点的氧化还原状态的动态变化和蛋白质丰度变化进行大规模并行分析。在这里，我们介绍FASILOX，这是GELSILOX方法的进一步发展，其特点是：i）显着提高了肽的回收率； ii）提高了检测Cys氧化变化的灵敏度，并且iii）简化了工作流程，从而缩短了检测时间。在从肥胖，糖尿病患者的脂肪组织中分离出的线粒体中，FASILOX揭示了Cys氧化的性二态性，主要涉及线粒体氧化磷酸化复合体。