Abstract Commercial ingredients containing milk fat globule membrane (MFGM) material are currently isolated from heavily processed dairy streams. The aim of this study was to achieve a more gentle isolation of MFGM material by means of ceramic dia-microfiltration of raw whole milk to separate fat globules from casein micelles and whey proteins prior to MFGM extraction. A pilot-scale experiment with 1.4 μm pore size (membrane surface area 1.05 m2) resulted in an optimal outcome of low permeation of fat (2.5% permeation) and high permeation of proteins (97% permeation). This yielded an MFGM isolate with 7% w/w polar lipids and 30% w/w proteins, where contamination of non-MFGM proteins was only 25% of total protein content. Furthermore, mild pasteurization (72 °C, 15 s) introduced either before or after microfiltration had no impact on filtration efficiency or MFGM yield and composition. The work describes an industrially relevant production method for a less-processed MFGM material of high purity with potential for further separation and valorisation of protein-rich permeate streams.

中文翻译：

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原奶微滤生产高纯乳脂肪球膜材料

摘要 含有乳脂肪球膜 (MFGM) 材料的商业成分目前是从经过大量加工的乳制品中分离出来的。本研究的目的是在 MFGM 提取之前，通过对生全脂牛奶进行陶瓷直径微滤以将脂肪球与酪蛋白胶束和乳清蛋白分离，从而实现更温和的 MFGM 材料分离。具有 1.4 μm 孔径（膜表面积 1.05 m2）的中试规模实验产生了低脂肪渗透（2.5% 渗透）和高蛋白质渗透（97% 渗透）的最佳结果。这产生了具有 7% w/w 极性脂质和 30% w/w 蛋白质的 MFGM 分离物，其中非 MFGM 蛋白质的污染仅占总蛋白质含量的 25%。此外，温和的巴氏杀菌（72°C，15 s) 在微滤之前或之后引入对过滤效率或 MFGM 产量和组成没有影响。这项工作描述了一种工业相关的生产方法，用于生产加工较少的高纯度 MFGM 材料，具有进一步分离和提高富含蛋白质的渗透物流的潜力。