BACKGROUND Patchouli alcohol (PA) is a tricyclic sesquiterpene extracted from Pogostemonis Herba, which is a traditional Chinese medicine used for therapy of inflammatory diseases. Recent studies have shown that PA has various pharmacological activities, including anti-bacterial and anti-viral effects. METHODS In this study, the anti-influenza virus (IAV) activities and mechanisms were investigated both in vitro and in vivo. The inhibitory effects of PA against IAV in vitro were evaluated by plaque assay and immunofluorescence assay. The neuraminidase inhibition assay, hemagglutination inhibition (HI) assay, and western blot assay were used to explore the anti-viral mechanisms. The anti-IAV activities in vivo were determined by mice pneumonia model and HE staining. RESULTS The results showed that PA significantly inhibited different IAV strains multiplication in vitro, and may block IAV infection through inactivating virus particles directly and interfering with some early stages after virus adsorption. Cellular PI3K/Akt and ERK/MAPK signaling pathways may be involved in the anti-IAV actions of PA. Intranasal administration of PA markedly improved mice survival and attenuated pneumonia symptoms in IAV infected mice, comparable to the effects of Oseltamivir. CONCLUSIONS Therefore, Patchouli alcohol has the potential to be developed into a novel anti-IAV agent in the future.

中文翻译：

---

广patch香醇通过靶向细胞PI3K / Akt和ERK / MAPK信号通路对甲型流感病毒的抑制作用。

背景技术广Pat香醇（PA）是从五味子中提取的三环倍半萜烯，其是用于治疗炎性疾病的传统中药。最近的研究表明，PA具有多种药理活性，包括抗菌和抗病毒作用。方法在本研究中，对体内和体外的抗流感病毒（IAV）活性和机理进行了研究。通过噬斑测定和免疫荧光测定评价PA在体外对IAV的抑制作用。使用神经氨酸酶抑制试验，血凝抑制（HI）试验和western印迹试验来探讨抗病毒机制。通过小鼠肺炎模型和HE染色确定体内抗IAV活性。结果结果表明，PA在体外显着抑制了不同的IAV病毒株的繁殖，并可能通过直接灭活病毒颗粒并干扰病毒吸附后的某些早期阶段来阻止IAV感染。细胞PI3K / Akt和ERK / MAPK信号通路可能参与PA的抗IAV作用。与Oseltamivir的作用相当，鼻内给药PA可以显着改善IAV感染小鼠的存活率并减轻肺炎症状。结论因此，广Pat香醇有潜力在将来被开发成新型的抗IAV剂。细胞PI3K / Akt和ERK / MAPK信号通路可能参与PA的抗IAV作用。与Oseltamivir的作用相当，鼻内给药PA可以显着改善IAV感染小鼠的存活率并减轻肺炎症状。结论因此，广Pat香醇有潜力在将来被开发成新型的抗IAV剂。细胞PI3K / Akt和ERK / MAPK信号通路可能参与PA的抗IAV作用。与Oseltamivir的作用相当，鼻内给药PA可以显着改善IAV感染小鼠的存活率并减轻肺炎症状。结论因此，广Pat香醇有潜力在将来被开发成新型的抗IAV剂。