Background Over the years, long non-coding RNAs (lncRNAs) have been clarified in malignancies, this research was focused on the role of lncRNA cartilage injury-related (lncRNA-CIR) in osteosarcoma cells. Methods LncRNA-CIR expression in osteosarcoma tissues and cells, and adjacent normal tissues and normal osteoblasts was determined, then the relations between lncRNA-CIR expression and the clinicopathological features, and between lncRNA-CIR expression and the prognosis of osteosarcoma patients were analyzed. Moreover, the MG63 and 143B cells were treated with silenced or overexpressed lncRNA-CIR, and then the proliferation, invasion, migration and apoptosis of the cells were evaluated by gain- and loss-of-function approaches. The tumor growth, and proliferation and apoptosis of osteosarcoma cells in vivo were observed by subcutaneous tumorigenesis in nude mice. Results We have found that lncRNA-CIR was up-regulated in osteosarcoma tissues and cells, which was respectively relative to adjacent normal tissues and normal osteoblasts. The expression of lncRNA-CIR was evidently correlated with disease stages, distant metastasis and differentiation of osteosarcoma patients, and the high expression of lncRNA-CIR indicated a poor prognosis. Furthermore, the reduction of lncRNA-CIR could restrict proliferation, invasion and migration, but promote apoptosis of osteosarcoma cells in vitro. Meanwhile, inhibited lncRNA-CIR also restrained tumor growth and osteosarcoma cell proliferation, whereas accelerated apoptosis of osteosarcoma cells in vivo. Conclusion We have found in this study that the inhibited lncRNA-CIR could decelerate proliferation, invasion and migration, but accelerate apoptosis of osteosarcoma cells, which may provide a novel target for osteosarcoma treatment.

中文翻译：

---

长链非编码 RNA-CIR 的降解减缓了骨肉瘤细胞的增殖、侵袭和迁移，但促进了骨肉瘤细胞的凋亡。

背景 多年来，长链非编码 RNA（lncRNAs）在恶性肿瘤中的作用已得到阐明，本研究重点关注 lncRNA 软骨损伤相关（lncRNA-CIR）在骨肉瘤细胞中的作用。方法测定骨肉瘤组织和细胞、癌旁正常组织和正常成骨细胞中lncRNA-CIR的表达，分析lncRNA-CIR表达与骨肉瘤患者临床病理特征、lncRNA-CIR表达与预后的关系。此外，MG63和143B细胞用沉默或过表达的lncRNA-CIR处理，然后通过功能获得和功能丧失方法评估细胞的增殖、侵袭、迁移和凋亡。肿瘤生长，裸鼠皮下成瘤观察体内骨肉瘤细胞的增殖和凋亡。结果我们发现lncRNA-CIR在骨肉瘤组织和细胞中上调，分别与邻近正常组织和正常成骨细胞相关。lncRNA-CIR的表达与骨肉瘤患者的疾病分期、远处转移和分化程度明显相关，lncRNA-CIR高表达提示预后不良。此外，lncRNA-CIR的降低可以限制体外骨肉瘤细胞的增殖、侵袭和迁移，但会促进骨肉瘤细胞的凋亡。同时，抑制lncRNA-CIR也抑制了肿瘤生长和骨肉瘤细胞增殖，同时加速了体内骨肉瘤细胞的凋亡。