Influence of Fiber Stiffness on Meniscal Cell Migration into Dense Fibrous Networks.,Advanced Healthcare Materials

当前位置： X-MOL 学术 › Adv. Healthcare Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Influence of Fiber Stiffness on Meniscal Cell Migration into Dense Fibrous Networks.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2019-12-23 , DOI: 10.1002/adhm.201901228
Kwang Hoon Song _{1,

2} , Su-Jin Heo _{2,

3,

4} , Ana P Peredo _{1,

2,

3,

4} , Matthew D Davidson _{1,

2} , Robert L Mauck _{1,

2,

3,

4} , Jason A Burdick _{1,

2,

4}

Affiliation

Fibrous scaffolds fabricated via electrospinning are being explored to repair injuries within dense connective tissues. However, there is still much to be understood regarding the appropriate scaffold properties that best support tissue repair. In this study, the influence of the stiffness of electrospun fibers on cell invasion into fibrous scaffolds is investigated. Specifically, soft and stiff electrospun fibrous networks are fabricated from crosslinked methacrylated hyaluronic acid (MeHA), where the stiffness is altered via the extent of MeHA crosslinking. Meniscal fibrochondrocyte (MFC) adhesion and migration into fibrous networks are investigated, where the softer MeHA fibrous networks are easily deformed and densified through cellular tractions and the stiffer MeHA fibrous networks support ≈50% greater MFC invasion over weeks when placed adjacent to meniscal tissue. When the scaffolds are sandwiched between meniscal tissues and implanted subcutaneously, the stiffer MeHA fibrous networks again support enhanced cellular invasion and greater collagen deposition after 4 weeks when compared to the softer MeHA fibrous networks. These results indicate that the mechanics and deformability of fibrous networks likely alter cellular interactions and invasion, providing an important design parameter toward the engineering of scaffolds for tissue repair.

中文翻译：

纤维刚度对半月板细胞迁移到致密纤维网络的影响。

人们正在探索通过静电纺丝制造的纤维支架来修复致密结缔组织内的损伤。然而，关于最能支持组织修复的适当支架特性，仍有很多需要了解。在本研究中，研究了电纺纤维的刚度对细胞侵袭纤维支架的影响。具体来说，柔软和坚硬的静电纺丝纤维网络由交联甲基丙烯酸透明质酸 (MeHA) 制成，其中硬度通过 MeHA 交联程度来改变。研究了半月板纤维软骨细胞 (MFC) 粘附和迁移到纤维网络中的情况，其中较软的 MeHA 纤维网络很容易通过细胞牵引变形和致密化，而较硬的 MeHA 纤维网络当放置在半月板组织附近时，可在数周内支持约 50% 的 MFC 侵袭。当支架夹在半月板组织之间并植入皮下时，与较软的 MeHA 纤维网络相比，4 周后，较硬的 MeHA 纤维网络再次支持增强的细胞侵袭和更多的胶原蛋白沉积。这些结果表明，纤维网络的力学和可变形性可能会改变细胞相互作用和侵袭，为组织修复支架工程提供重要的设计参数。

更新日期：2020-04-21

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11