Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury.,Biomaterials Science

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Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-01-06 , DOI: 10.1039/c9bm00901a
Ana Catarina Pinho ₁ , Mariana Vieira Branquinho ₂ , Rui Damásio Alvites ₂ , Ana Clotilde Fonseca ₁ , Ana Rita Caseiro ₃ , Sílvia Santos Pedrosa ₂ , Ana Lúcia Luís ₂ , Isabel Pires ₄ , Justina Prada ₄ , Luísa Muratori ₅ , Giulia Ronchi ₅ , Stefano Geuna ₄ , José Domingos Santos ₆ , Ana Colette Maurício ₂ , Arménio Coimbra Serra ₁ , Jorge Fernando Jordão Coelho ₁

Affiliation

CEMMPRE, Department of Chemical Engineering, Rua Sílvio Lima-Pólo II, 3030-790 Coimbra, Portugal.
Veterinary Clinics Department, Abel Salazar Biomedical Sciences Institute (ICBAS), University of Porto (UP), Rua de Jorge Viterbo Ferreira, no. 228, 4050-313 Porto, Portugal and Animal Science Study Centre (CECA), University of Porto Agroenvironment, Technologies and Sciences Institute (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.
Veterinary Clinics Department, Abel Salazar Biomedical Sciences Institute (ICBAS), University of Porto (UP), Rua de Jorge Viterbo Ferreira, no. 228, 4050-313 Porto, Portugal and Animal Science Study Centre (CECA), University of Porto Agroenvironment, Technologies and Sciences Institute (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal and Vasco da Gama University School/Escola Universitária Vasco da Gama (EUVG), Av. José R. Sousa Fernandes 197, Campus Universitário - Bloco B, Lordemão, 3020-210 Coimbra, Portugal.
CECAV and Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal.
Neuroscience Institute of the Cavalieri Ottolenghi Foundation and Department of Clinical and Biological Sciences, University of Turin, 10043 Turin, Italy.
REQUIMTE-LAQV, Department of Metallurgy and Materials, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias s/n, 4200-465 Porto, Portugal.

In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous and porous transparent tube-guides were obtained by photocrosslinking of two co-macromonomers based on dextran and poly(ε-caprolactone) (PCL). Swelling capacity of the tube-guides ranged from 40-60% with no visible constriction of their inner diameter. In vitro hydrolytic degradation tests showed that the tube-guides maintained their structural integrity up to 6 months. The in vivo performance of the tube-guides was evaluated by entubulation of the rat sciatic nerve after a neurotmesis injury, with a 10 mm-gap between the nerve stumps. The results showed that the tube-guides were able to promote the regeneration of the nerve in a similar manner to what was observed with conventional techniques (nerve graft and end-to-end suture). Stereological analysis proved that nerve regeneration occurred, and both tube-guides presented fibre diameter and g-ratio closer to healthy sciatic nerves. The histomorphometric analysis of Tibialis anterior (TA) skeletal muscle showed decreased neurogenic atrophy in the porous tube-guides treated group, presenting measurements that are similar to the uninjured control.

中文翻译：

基于右旋糖酐的管引导剂，用于神经支气管损伤后大鼠坐骨神经的再生。

在这项工作中，基于葡聚糖的神经管导管已被制备，表征并用于神经性眼病损伤的标准化动物模型中。通过基于葡聚糖和聚（ε-己内酯）（PCL）的两种共聚大分子单体的光交联，获得了无孔和多孔的透明导管。导管的膨胀能力在40％至60％之间，其内径没有明显的收缩。体外水解降解试验表明，导管可保持其结构完整性长达6个月。通过在神经分裂伤后对大鼠坐骨神经进行插管，在神经残端之间留有10 mm的间隙，对导管的体内性能进行评估。结果表明，导管导向器能够以与常规技术（神经移植和端对端缝合）相似的方式促进神经再生。体视学分析证明发生了神经再生，并且两种导管均显示纤维直径和g比值更接近健康的坐骨神经。胫骨前（TA）骨骼肌的组织形态计量学分析显示，在多孔导管治疗组中神经原性萎缩减少，其测量结果与未受伤的对照组相似。

更新日期：2020-02-13

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