Abstract Holder pasteurization is a thermal treatment commonly applied to milk in human milk banks. However, the application of heating processes can affect the actual composition of milk. In this work, the effect of Holder pasteurization on essential elements in donor human breast milk was studied, including total contents and protein-binding profile of Cu, Fe, Se and Zn. The total contents were determined by inductively coupled plasma mass spectrometry (ICP-MS) and chemical speciation studies were conducted by size exclusion chromatography (SEC) coupled to an ICP-MS and to an UV–vis detector. Total concentrations (μg/L) of Cu (0.19 ± 0.01), Fe (0.35 ± 0.01), Se (0.010 ± 0.003) and Zn (0.57 ± 0.01) were obtained before pasteurization and concentrations of Cu (0.19 ± 0.01), Fe (0.36 ± 0.02), Se (0.008 ± 0.002) and Zn (0.57 ± 0.01) were obtained after pasteurization. Their protein-binding profiles did not change significantly for the majority of the species, indicating a low degree of interconversion due to the elevation of the temperature. However, protein profiles changed after pasteurization, indicating that the losses occurred mainly in the apo form (metal-free form) of proteins rather than in their holo forms (metallo-species).

中文翻译：

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Holder 巴氏杀菌对人类捐赠乳中必需元素的影响：总含量和蛋白质结合特征

摘要 巴氏杀菌是一种常用于母乳库中牛奶的热处理。然而，加热过程的应用会影响牛奶的实际成分。在这项工作中，研究了霍尔德巴氏杀菌对供体母乳中必需元素的影响，包括铜、铁、硒和锌的总含量和蛋白质结合特征。总含量通过电感耦合等离子体质谱 (ICP-MS) 测定，化学形态研究通过与 ICP-MS 和 UV-vis 检测器耦合的尺寸排阻色谱 (SEC) 进行。在巴氏杀菌之前获得了 Cu (0.19 ± 0.01)、Fe (0.35 ± 0.01)、Se (0.010 ± 0.003) 和 Zn (0.57 ± 0.01) 的总浓度 (μg/L) 以及 Cu (0.19 ± 0.01)、Fe 的浓度(0.36 ± 0.02)、Se (0.008 ± 0.002) 和 Zn (0.57 ± 0. 01) 在巴氏杀菌后获得。对于大多数物种，它们的蛋白质结合谱没有显着变化，表明由于温度升高，相互转化程度较低。然而，巴氏杀菌后蛋白质谱发生了变化，表明损失主要发生在蛋白质的 apo 形式（无金属形式）而不是它们的全息形式（金属物种）中。