Three-dimensional (3D) printing technology offers attractive possibilities for many fields. In electrochemistry, 3D printing technology has been used to fabricate customized 3D-printed electrodes as a platform to develop bio/sensing, energy generation and storage devices. Here, we use a 3D-printed graphene/polylactic (PLA) electrode made by additive manufacturing technology and immobilize horseradish peroxidase (HRP) to create a direct electron transfer enzyme-based biosensors for hydrogen peroxide detection. Gold nanoparticles are included in the system to confirm and facilitate heterogeneous electron transfer. This work opens a new direction for the fabrication of third-generation electrochemical biosensors using 3D printing technology, with implications for applications in the environmental and biomedical fields.

中文翻译：

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3D打印的石墨烯直接电子转移酶生物传感器。

三维（3D）打印技术为许多领域提供了诱人的可能性。在电化学领域，3D打印技术已用于制造定制的3D打印电极，以此作为开发生物/传感，能量产生和存储设备的平台。在这里，我们使用通过增材制造技术制造的3D打印石墨烯/聚乳酸（PLA）电极，并固定辣根过氧化物酶（HRP）来创建用于过氧化氢检测的基于直接电子转移酶的生物传感器。金纳米颗粒包括在系统中，以确认并促进异质电子转移。这项工作为使用3D打印技术制造第三代电化学生物传感器开辟了新的方向，对环境和生物医学领域的应用产生了影响。