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Single-cell analysis of human adipose tissue identifies depot and disease specific cell types.
Nature Metabolism ( IF 18.9 ) Pub Date : 2019-12-23 , DOI: 10.1038/s42255-019-0152-6
Jinchu Vijay 1, 2 , Marie-Frédérique Gauthier 3 , Rebecca L Biswell 4 , Daniel A Louiselle 4 , Jeffrey J Johnston 4 , Warren A Cheung 4 , Bradley Belden 4 , Albena Pramatarova 2 , Laurent Biertho 3 , Margaret Gibson 4 , Marie-Michelle Simon 2 , Haig Djambazian 2 , Alfredo Staffa 2 , Guillaume Bourque 1, 2 , Anita Laitinen 5 , Johanna Nystedt 5 , Marie-Claude Vohl 6 , Jason D Fraser 7 , Tomi Pastinen 4 , André Tchernof 3 , Elin Grundberg 4
Affiliation  

The complex relationship between metabolic disease risk and body fat distribution in humans involves cellular characteristics which are specific to body fat compartments. Here we show depot-specific differences in the stromal vascual fraction of visceral and subcutaneous adipose tissue by performing single-cell RNA sequencing of tissue specimen from obese individuals. We characterize multiple immune cells, endothelial cells, fibroblasts, adipose and hematopoietic stem cell progenitors. Subpopulations of adipose-resident immune cells are metabolically active and associated with metabolic disease status and those include a population of potential dysfunctional CD8+ T cells expressing metallothioneins. We identify multiple types of adipocyte progenitors that are common across depots, including a subtype enriched in individuals with type 2 diabetes. Depot-specific analysis reveals a class of adipocyte progenitors unique to visceral adipose tissue, which shares common features with beige preadipocytes. Our human single-cell transcriptome atlas across fat depots provides a resource to dissect functional genomics of metabolic disease.

中文翻译:

人体脂肪组织的单细胞分析可识别贮库和疾病特异性细胞类型。

代谢疾病风险与人体脂肪分布之间的复杂关系涉及特定于身体脂肪区室的细胞特征。在这里,我们通过对来自肥胖个体的组织样本进行单细胞 RNA 测序,显示内脏和皮下脂肪组织的基质血管部分的仓库特异性差异。我们表征了多种免疫细胞、内皮细胞、成纤维细胞、脂肪和造血干细胞祖细胞。脂肪驻留免疫细胞亚群具有代谢活性并与代谢疾病状态相关,其中包括表达金属硫蛋白的潜在功能失调 CD8+ T 细胞群。我们确定了多种类型的脂肪细胞祖细胞,这些祖细胞在仓库中很常见,包括富含 2 型糖尿病患者的亚型。仓库特异性分析揭示了内脏脂肪组织特有的一类脂肪细胞祖细胞,其与米色前脂肪细胞具有共同特征。我们跨脂肪库的人类单细胞转录组图谱为剖析代谢疾病的功能基因组学提供了资源。
更新日期:2019-12-23
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