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Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells.
Nature Biotechnology ( IF 33.1 ) Pub Date : 2019-12-23 , DOI: 10.1038/s41587-019-0359-9
Miguel A Pleitez 1, 2 , Asrar Ali Khan 3, 4, 5, 6 , Alice Soldà 1, 2 , Andriy Chmyrov 1, 2 , Josefine Reber 1, 2 , Francesca Gasparin 1, 2 , Markus R Seeger 1, 2 , Benedikt Schätz 1, 2 , Stephan Herzig 3, 4, 5, 6 , Marcel Scheideler 3, 4, 5, 6 , Vasilis Ntziachristos 1, 2
Affiliation  

We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.

中文翻译:

在活细胞中通过中红外光声显微镜进行无标记的代谢成像。

我们开发了中红外光声显微镜(MiROM),用于无标记,键选择,活细胞代谢成像,能够时空监测细胞和组织中的碳水化合物,脂质和蛋白质。通过对光吸收的声学检测,MiROM将中红外感应转换为正对比度成像方式,其光损伤可忽略不计,并且具有很高的灵敏度。我们使用MiROM来观察在脂肪形成过程中内在碳水化合物分布的变化,从扩散的空间模式到与脂滴的紧密共定位。
更新日期:2019-12-23
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