Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells.,Nature Biotechnology

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Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells.
Nature Biotechnology ( IF 33.1 ) Pub Date : 2019-12-23 , DOI: 10.1038/s41587-019-0359-9
Miguel A Pleitez _{1,

2} , Asrar Ali Khan _{3,

4,

5,

6} , Alice Soldà _{1,

2} , Andriy Chmyrov _{1,

2} , Josefine Reber _{1,

2} , Francesca Gasparin _{1,

2} , Markus R Seeger _{1,

2} , Benedikt Schätz _{1,

2} , Stephan Herzig _{3,

4,

5,

6} , Marcel Scheideler _{3,

4,

5,

6} , Vasilis Ntziachristos _{1,

2}

Affiliation

We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.

中文翻译：

在活细胞中通过中红外光声显微镜进行无标记的代谢成像。

我们开发了中红外光声显微镜（MiROM），用于无标记，键选择，活细胞代谢成像，能够时空监测细胞和组织中的碳水化合物，脂质和蛋白质。通过对光吸收的声学检测，MiROM将中红外感应转换为正对比度成像方式，其光损伤可忽略不计，并且具有很高的灵敏度。我们使用MiROM来观察在脂肪形成过程中内在碳水化合物分布的变化，从扩散的空间模式到与脂滴的紧密共定位。

更新日期：2019-12-23

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