Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular proteins.,Nature Biotechnology

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Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular proteins.
Nature Biotechnology ( IF 33.1 ) Pub Date : 2019-12-23 , DOI: 10.1038/s41587-019-0372-z
Jase Gehring _{1,

2} , Jong Hwee Park ₂ , Sisi Chen ₂ , Matthew Thomson ₂ , Lior Pachter _{2,

3}

Affiliation

We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cells are chemically labeled by attaching identifying DNA oligonucleotides to cellular proteins. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing an efficient method for surveying cell populations from large experiments or clinical samples with the depth and resolution of single-cell RNA sequencing.

中文翻译：

通过细胞蛋白的DNA寡核苷酸标记高度复用的单细胞RNA-seq。

我们描述了一种用于单细胞RNA测序的通用样品多路复用方法，其中通过将鉴定DNA寡核苷酸附着到细胞蛋白上来对固定细胞进行化学标记。对96重扰动实验的分析揭示了无法从批量测量中识别出的细胞群体结构和转录状态的变化，从而建立了一种有效的方法，可通过深度和单细胞RNA测序的分辨率对大型实验或临床样品中的细胞群体进行调查。

更新日期：2019-12-23

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