Gangliosides (GG) are glycosphingolipids, composed of a ceramide moiety (fatty acid and long chain base) linked to an oligosaccharide chain containing one (or more) molecule of sialic acid. After lipid extraction from biological matrices, quantification of GG by liquid chromatography coupled to electrospray ionization mass spectrometry (LC-ESI/MS) can be impacted by ion suppression effects due to co-elution with more abundant lipids in the matrix. In this study, a simple, rapid and efficient method to purify GG from biological samples by Phree columns is proposed. This approach proved to be useful in eliminating phospholipids (PL) from the matrix and thus increasing the signal of GG classes and molecular species in rat brain samples during LC-ESI/MS analysis.

神经节苷脂（GG）是糖鞘脂，由神经酰胺部分（脂肪酸和长链碱基）连接到含有一个（或多个）唾液酸分子的寡糖链上。从生物基质中提取脂质后，由于液相色谱与电喷雾电离质谱联用（LC-ESI / MS）进行的GG定量分析可能会受到离子抑制作用的影响，这是因为与基质中含量更高的脂质共洗脱所致。在这项研究中，提出了一种通过Phree色谱柱从生物样品中纯化GG的简单，快速，有效的方法。在LC-ESI / MS分析过程中，该方法被证明可用于消除基质中的磷脂（PL），从而增加大鼠脑样品中GG类和分子物质的信号。