Three undescribed chromanones (1-3) and two known chromanones (4 and 5) were isolated from the culture of endophytic fungus Phomopsis CGMCC No. 5416 from the stems of Achyranthes bidentata. Their structures were determined by spectroscopic analysis. Compounds 1 and 2 showed inhibitory activities against HIV-1 with IC50 values of 20.4 and 32.5 μg ml-1, respectively. Compounds 1-3 displayed moderate cytotoxicity with CC50 values of 36.5-79.3 μg ml-1 against A549, MDA-MB-231, and PANC-1 cell lines. Moreover, compound 3 can induce the early apoptosis of PANC-1 cancer cells with the apoptosis rate of 10.52%.

中文翻译：

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来自内生真菌Phomopsis CGMCC No.5416的三种具有生物活性的新发色酮。

从牛膝草的茎中从内生真菌Phomopsis CGMCC No.5416的培养物中分离出三个未描述的苯并二氢吡喃酮（1-3）和两个已知的苯并二氢吡喃酮（4和5）。通过光谱分析确定它们的结构。化合物1和2对HIV-1表现出抑制活性，IC50值分别为20.4和32.5μgml-1。化合物1-3对A549，MDA-MB-231和PANC-1细胞系显示出中等的细胞毒性，CC50值为36.5-79.3μgml-1。此外，化合物3可以诱导PANC-1癌细胞的早期凋亡，其凋亡率为10.52％。