Key Points Twelve-hour cDC1 IL-27p28 expression predicts adjuvant-elicited CD8 T cell memory. cDC1 IL-27p28 expression uniquely stratifies T cell–inducing adjuvants. Visual Abstract Although adjuvants and formulations are often either empirically derived, or at best judged by their ability to elicit broad inflammation, it would be ideal if specific innate correlates of adaptive immunity could be identified to set a universally applicable benchmark for adjuvant evaluation. Using an IL-27 reporter transgenic mouse model, we show in this study that conventional type 1 dendritic cell IL-27 production in the draining lymph node 12 h after s.c. vaccination directly correlates with downstream CD8+ T cell memory and protective immunity against infectious challenge. This correlation is robust, reproducible, predictive, entirely unique to vaccine biology, and is the only innate correlate of CD8+ T cell immune memory yet to be identified. Our results provide new insights into the basic biology of adjuvant-elicited cellular immunity and have clear implications for the screening and evaluation of novel adjuvants.

中文翻译：

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cDC1 IL-27p28 的产生可预测疫苗引发的 CD8+ T 细胞记忆和保护性免疫

关键点 12 小时 cDC1 IL-27p28 表达预测佐剂引发的 CD8 T 细胞记忆。cDC1 IL-27p28 表达独特地分层 T 细胞诱导佐剂。视觉摘要 尽管佐剂和制剂通常是根据经验得出的，或者最多可以通过它们引起广泛炎症的能力来判断，但如果可以识别出适应性免疫的特定先天相关性来为佐剂评估设定普遍适用的基准，那将是理想的。使用 IL-27 报告基因转基因小鼠模型，我们在这项研究中表明，皮下接种后 12 小时引流淋巴结中常规 1 型树突状细胞 IL-27 的产生与下游 CD8+ T 细胞记忆和针对感染性挑战的保护性免疫直接相关。这种相关性是强大的、可重复的、可预测的，完全是疫苗生物学独有的，并且是唯一尚未确定的 CD8+ T 细胞免疫记忆的先天相关性。我们的研究结果为佐剂引发的细胞免疫的基本生物学提供了新的见解，并对新型佐剂的筛选和评估具有明确的意义。