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Cancer Cell Membrane Vesicle for Multiplex MicroRNA Imaging in Living Cells.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-12-23 , DOI: 10.1021/acs.analchem.9b03764 Huiting Lu 1 , Keke Guo 1 , Yu Cao 1, 2 , Fan Yang 1 , Dongdong Wang 1 , Lei Dou 3 , Yayun Liu 4 , Haifeng Dong 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-12-23 , DOI: 10.1021/acs.analchem.9b03764 Huiting Lu 1 , Keke Guo 1 , Yu Cao 1, 2 , Fan Yang 1 , Dongdong Wang 1 , Lei Dou 3 , Yayun Liu 4 , Haifeng Dong 1
Affiliation
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Highly efficient cellular transfection and intracellular signal amplification is a prerequisite for low-abundant microRNA (miRNA) imaging and biomedical application. Herein, we report a functional cancer cell membrane (CM) vesicle, Au-P/DSN@CM (DSN, double-specific nucleases), which consists of Au nanoparticles modified with three types of fluorescent miRNA detection probes (Au-P) and DSN that simultaneously encapsulate in cancer CM. We find that the Au-P/DSN@CM could specifically target the cancer cell and transfect the cell with higher efficiency than Au nanoparticles. The internalized Au-P/DSN@CM could further specifically recognize the target miRNA and induce DSN-assisted target recycle signal amplification, leading to multiple miRNA simultaneous detection with high sensitivity. It successfully detects oncogenic miRNAs in MCF-7 cells with high sensitivity and is amenable to monitor the dynamic expression change of oncogenic miRNAs in cancer cells. Our study represents a promising gene delivery vector for cancer diagnosis and potential therapy.
中文翻译：
在活细胞中进行多重MicroRNA成像的癌细胞膜囊泡。
高效的细胞转染和细胞内信号放大是低丰度microRNA（miRNA）成像和生物医学应用的先决条件。在这里，我们报告功能性癌细胞膜（CM）囊泡，Au-P / DSN @ CM（DSN，双特异性核酸酶），其由用三种类型的荧光miRNA检测探针（Au-P）和同时封装在癌症CM中的DSN。我们发现，Au-P / DSN @ CM可以特异性靶向癌细胞并以比Au纳米颗粒更高的效率转染细胞。内在化的Au-P / DSN @ CM可以进一步特异性识别目标miRNA，并诱导DSN辅助的目标回收信号扩增，从而以高灵敏度同时检测多个miRNA。它能够以高灵敏度成功检测MCF-7细胞中的致癌miRNA，并且可监测癌细胞中致癌miRNA的动态表达变化。我们的研究代表了用于癌症诊断和潜在治疗的有前途的基因传递载体。
更新日期：2020-01-09
中文翻译：
在活细胞中进行多重MicroRNA成像的癌细胞膜囊泡。
高效的细胞转染和细胞内信号放大是低丰度microRNA（miRNA）成像和生物医学应用的先决条件。在这里，我们报告功能性癌细胞膜（CM）囊泡，Au-P / DSN @ CM（DSN，双特异性核酸酶），其由用三种类型的荧光miRNA检测探针（Au-P）和同时封装在癌症CM中的DSN。我们发现，Au-P / DSN @ CM可以特异性靶向癌细胞并以比Au纳米颗粒更高的效率转染细胞。内在化的Au-P / DSN @ CM可以进一步特异性识别目标miRNA，并诱导DSN辅助的目标回收信号扩增，从而以高灵敏度同时检测多个miRNA。它能够以高灵敏度成功检测MCF-7细胞中的致癌miRNA，并且可监测癌细胞中致癌miRNA的动态表达变化。我们的研究代表了用于癌症诊断和潜在治疗的有前途的基因传递载体。
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