A composite prepared from MoS2 quantum dots and silver nanoparticles and stimulated by mercury(II) is a robust oxidase mimetic for use in visual determination of cysteine,Microchimica Acta

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A composite prepared from MoS2 quantum dots and silver nanoparticles and stimulated by mercury(II) is a robust oxidase mimetic for use in visual determination of cysteine
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-12-20 , DOI: 10.1007/s00604-019-4041-1
Ravi Prakash Ojha ₁ , Richa Mishra ₁ , Priya Singh ₁ , Narsingh Raw Nirala ₁ , Rajiv Prakash ₁

Affiliation

MoS2 quantum dots were hydrothermally synthesized and utilized for the formation and stabilization of a nanocomposite with silver nanoparticles (AgNPs) in a single step. This composite was characterized by transmission electron microscopy and zeta potential measurements. It is found that this nanohybrid can be stimulated by mercury(II) ion and then exhibits excellent oxidase mimicking activity. The oxidase-like activity is demonstrated by the oxidation of 3,3′,5,5′-tetramethylbenzidine by H2O2 that leads to the formation of a blue product. An assay was developed for determination of cysteine (Cys) at ultra-trace level because Cys inhibits the activity of the nanozyme via interaction with Hg(II). The Cys assay, best performed at a wavelength of 652 nm, works in the 1–100 μM concentration range and has a 0.82 μM detection limit. In addition, a portable Cys test kit is described that was applied to the determination of Cys in serum samples. The resulting colorations were compared with color chat wheel. The method is simple, rapid, cost-effective, and sensitive. Graphical abstract Schematic presentation of oxidase mimetic activity of the Hg@ MoS2-QDs-AgNPs and colorimetric sensing of Cys. Schematic presentation of oxidase mimetic activity of the Hg@ MoS2-QDs-AgNPs and colorimetric sensing of Cys.

中文翻译：

由二硫化钼量子点和银纳米粒子制备并受汞 (II) 刺激的复合材料是一种强大的氧化酶模拟物，可用于视觉测定半胱氨酸

MoS2 量子点是水热合成的，并用于一步形成和稳定与银纳米粒子 (AgNPs) 的纳米复合材料。这种复合材料的特征在于透射电子显微镜和 zeta 电位测量。发现这种纳米杂化物可以被汞（II）离子刺激，然后表现出优异的氧化酶模拟活性。氧化酶样活性通过 H2O2 氧化 3,3',5,5'-四甲基联苯胺证明，导致形成蓝色产物。开发了一种测定超痕量半胱氨酸 (Cys) 的方法，因为 Cys 通过与 Hg(II) 的相互作用抑制纳米酶的活性。Cys 检测最好在 652 nm 的波长下进行，在 1–100 μM 浓度范围内工作，检测限为 0.82 μM。此外，描述了一种便携式Cys检测试剂盒，用于测定血清样品中的Cys。将所得着色与彩色聊天轮进行比较。该方法简单、快速、经济、灵敏。Hg@MoS2-QDs-AgNPs 的氧化酶模拟活性和 Cys 的比色传感的图形摘要示意图。Hg@MoS2-QDs-AgNPs 的氧化酶模拟活性和 Cys 的比色传感示意图。

更新日期：2019-12-20

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