当前位置：
X-MOL 学术
›
ACS Appl. Polym. Mater.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Catechol-Attached Polypeptide with Functional Groups as Electrochemical Sensing Platform for Synthetic Cannabinoids
ACS Applied Polymer Materials ( IF 4.4 ) Pub Date : 2019-12-31 , DOI: 10.1021/acsapm.9b00730 Ceren Durmus 1 , Eda Aydindogan 1 , Zinar Pinar Gumus 2 , Takeshi Endo 3 , Shuhei Yamada 3 , Hakan Coskunol 4 , Suna Timur 1, 2 , Yusuf Yagci 5, 6
ACS Applied Polymer Materials ( IF 4.4 ) Pub Date : 2019-12-31 , DOI: 10.1021/acsapm.9b00730 Ceren Durmus 1 , Eda Aydindogan 1 , Zinar Pinar Gumus 2 , Takeshi Endo 3 , Shuhei Yamada 3 , Hakan Coskunol 4 , Suna Timur 1, 2 , Yusuf Yagci 5, 6
Affiliation
|
Herein, we first constructed a functional surface using a catechol-attached polypeptide (CtP) for the detection of JWH-018 (N-4-hydroxypentyl metabolite). K2 antibody was then incorporated to the polymer via covalent cross-linker. Step-by-step modifications on the glassy carbon electrode surface were characterized by electrochemical measurements such as differential pulse voltammetry, cyclic voltammetry, impedance spectroscopy, and X-ray photoelectron spectroscopy. Linearity and the limit of detection for JWH-018 (N-4-hydroxypentyl metabolite) were determined as 10–500 ng/mL with an equation of y = 0.0018x + 0.136 (R2 = 0.993) and 5.892 ng/mL, respectively. The selectivity of the biosensor was evaluated with different interfering molecules (methamphetamine, codeine, and cocaine). Finally, the biosensor was successfully used in the determination of JWH-018 (N-4-hydroxypentyl metabolite) in spiked synthetic urine samples, and a high-performance liquid chromatography (HPLC) system was used as a reference method to confirm the sample application. The results show that this biosensor platform can be applied to detect other JWH series of synthetic cannabinoids with high sensitivity and accuracy.
中文翻译：
具有功能基团的邻苯二酚连接多肽作为合成大麻素的电化学传感平台
在本文中，我们首先使用邻苯二酚连接的多肽（CtP）构建了功能表面，用于检测JWH-018（N -4-羟基戊基代谢产物）。然后将K2抗体通过共价交联剂掺入到聚合物中。通过电化学测量，例如差分脉冲伏安法，循环伏安法，阻抗谱和X射线光电子能谱，对玻璃碳电极表面的逐步修饰进行了表征。JWH-018（N -4-羟基戊基代谢产物）的线性和检出限确定为10–500 ng / mL，方程y = 0.0018 x + 0.136（R 2= 0.993）和5.892 ng / mL。用不同的干扰分子（甲基苯丙胺，可待因和可卡因）评估生物传感器的选择性。最后，该生物传感器已成功用于加标合成尿液样品中JWH-018（N -4-羟基戊基代谢物）的测定，并以高效液相色谱（HPLC）系统作为参考方法来确认样品的应用。结果表明，该生物传感器平台可用于检测其他JWH系列合成大麻素，具有较高的灵敏度和准确性。
更新日期：2019-12-31
中文翻译：
具有功能基团的邻苯二酚连接多肽作为合成大麻素的电化学传感平台
在本文中，我们首先使用邻苯二酚连接的多肽（CtP）构建了功能表面，用于检测JWH-018（N -4-羟基戊基代谢产物）。然后将K2抗体通过共价交联剂掺入到聚合物中。通过电化学测量，例如差分脉冲伏安法，循环伏安法，阻抗谱和X射线光电子能谱，对玻璃碳电极表面的逐步修饰进行了表征。JWH-018（N -4-羟基戊基代谢产物）的线性和检出限确定为10–500 ng / mL，方程y = 0.0018 x + 0.136（R 2= 0.993）和5.892 ng / mL。用不同的干扰分子（甲基苯丙胺，可待因和可卡因）评估生物传感器的选择性。最后，该生物传感器已成功用于加标合成尿液样品中JWH-018（N -4-羟基戊基代谢物）的测定，并以高效液相色谱（HPLC）系统作为参考方法来确认样品的应用。结果表明，该生物传感器平台可用于检测其他JWH系列合成大麻素，具有较高的灵敏度和准确性。
京公网安备 11010802027423号