Supraglacial debris of Miage Glacier (Mont Blanc, Italy) was used as an in situ model for monitoring growth and modification of the taxonomic structure of fungal populations using an in-growth mesh bag approach over three consecutive years. Sterilized debris was placed in mesh bags (MB) and buried in the debris layer. Pristine debris (D) covering the surface of the glacier was also studied for comparative purposes.

Quantitative PCR revealed that after a time of between one and two years the fungal abundance in the sterilized supraglacial debris contained in MB increased to reach a level comparable with those found in D samples. Likewise, the analysis of alpha-diversity exhibited similar dynamics during the three years of study. On the contrary, beta-diversity and Linear discriminant analysis Effect Size (LEfSe), showed apparently fluctuating dynamics from the first to the third year of study and a reduction of the number of fungal taxa discriminating MB and D samples. These observations confirm the hypothesis that fungal cells deposited on MB either by the surrounding debris or continuously brought via wind or atmospheric precipitations, can be subjected to an in situ growth which leads the abundance of the fungal communities occurring in MB to levels found in pristine debris (D) but showing fluctuating taxonomic structures.

Miage Glacier（意大利勃朗峰）的冰川上部碎片被用作就地模型，用于连续三年使用内生网袋方法监测真菌种群的生长和生物分类结构的改变。将消毒后的残渣放在网袋（MB）中，然后埋在残渣层中。为了比较，还研究了覆盖冰川表面的原始碎屑（D）。

定量PCR显示，经过一到两年的时间后，MB中包含的无菌上颌碎屑中的真菌丰度增加到了与D样品中发现的相当的水平。同样，在三年的研究过程中，对alpha多样性的分析也显示出相似的动态。相反，从研究的第一年到第三年，β多样性和线性判别分析效应量（LEfSe）表现出明显的波动动态，并且能够区分MB和D样品的真菌分类群数量减少了。这些观察结果证实了这样的假说，即可以将由周围碎片沉积或连续通过风或大气降水带入MB上的真菌细胞进行原位处理。 细菌的生长导致MB中出现的真菌群落的丰富度达到原始碎片（D）中的水平，但显示出波动的生物分类结构。