Population genetic studies play an integral role in understanding the ecology and management of fungal plant pathogens. Such studies for species of Botryosphaeriaceae are hampered by a lack of available markers. Genomic sequences are available for multiple species in this family and they provide excellent resources for the development of population genetic markers. Here we describe highly transferable microsatellite or simple sequence repeat (SSR) markers for species in Lasiodiplodia and Neofusicoccum; two important and globally distributed members of the Botryosphaeriaceae. These were developed by extracting SSR-containing sequences from available genomes. Seventy-seven markers were developed for Lasiodiplodia and 32 markers were developed for Neofusicoccum. Most of these markers were transferable between species within a genus. Twelve markers tested for fragment length polymorphism in 20 isolates of Lasiodiplodia mahajangana identified between two and nine alleles and gene diversities between 0.18 and 0.83. Eleven markers indicated between two and five alleles for 20 isolates of Neofusicoccum parvum and gene diversities between 0.26 and 0.57. The large number and high transferability of the developed markers will facilitate population studies of a wide range of Lasiodiplodia and Neofusicoccum species associated with tree diseases globally.

种群遗传学研究在理解真菌植物病原体的生态和管理方面起着不可或缺的作用。缺乏可用标记阻碍了对球形葡萄球菌物种的此类研究。基因组序列可用于该家族中的多个物种，它们为开发种群遗传标记提供了极好的资源。在这里，我们描述了Lasiodiplodia和Neofusicoccum中物种的高度可转移微卫星或简单序列重复（SSR）标记；Botryosphaeriaceae的两个重要且在全球分布的成员。这些是通过从可用基因组中提取含有SSR的序列而开发的。为Lasiodiplodia开发了77个标记并为新镰孢菌开发了32个标记。这些标记大多数都可以在属内的物种之间转移。测试了十二个Lasiodiplodia mahajangana分离株的片段长度多态性的十二个标记，鉴定出两个和九个等位基因之间，基因多样性在0.18和0.83之间。11个标记指示了20个细小镰刀菌分离株的2至5个等位基因之间，基因多样性在0.26和0.57之间。已开发的标记物的数量众多且具有很高的可移植性，将有助于全球范围内与树木病害相关的多种Lasiodiplodia和Neofusicoccum物种的种群研究。