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The neuropeptidome of Carabus (Coleoptera, Adephaga: Carabidae).
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2019-12-21 , DOI: 10.1016/j.ibmb.2019.103309
Lapo Ragionieri 1 , Reinhard Predel 1
Affiliation  

Neuropeptides are signaling molecules involved in the regulation of virtually all physiological functions of Metazoa. In insects, more than 50 neuropeptide genes can be present in a single species, and thus neuropeptidergic systems are attractive targets for the development of environmentally friendly pesticides. Such approaches require not only knowledge of the neuropeptidomes of pests, but also detailed knowledge of the corresponding systems in beneficial insects. In Coleoptera, there is no profound knowledge of the neuropeptides in the adephagan lineage, which contains many of the ecologically important predators of caterpillars. We analyzed by transcriptomics, mass spectrometry and immunohistochemistry the neuropeptidomes of the two Carabus species C. violaceus and C. problematicus. This information, which contains detailed data on the differential processing of CAPA peptides, allows for the recognition of features typical only of the polyphagan lineage with its many pests. The neuropeptidomics data, which also confirmed the processing of a number of protein hormones, represent the highest number of neuropeptides that have been identified so far from Coleoptera. The sequences of the mature neuropeptides of the two Carabus species, whose ancestors separated about 13 Mya, are highly similar and no sequence substitutions were found in single-copy neuropeptides.

中文翻译:

Carabus(鞘翅目,Adephaga:Carabidae)的神经肽组。

神经肽是信号分子,几乎参与调节后生动物的所有生理功能。在昆虫中,单个物种中可以存在50多种神经肽基因,因此,神经肽能系统是开发环保农药的诱人目标。这样的方法不仅需要了解害虫的神经肽,而且还需要了解有益昆虫中相应系统的详细知识。在鞘翅目中,对无足动物谱系中的神经肽没有深入的了解,无足蛋白谱系包含许多生态上重要的毛毛虫捕食者。我们通过转录组学,质谱和免疫组织化学分析了两个水瓶物种C. violaceus和C. problematicus的神经肽。这个信息,其中包含有关CAPA肽差异处理的详细数据,可以识别只有多噬菌体谱系及其许多害虫才具有的典型特征。神经肽组学数据也证实了许多蛋白激素的加工,代表了迄今为止从鞘翅目中发现的最多的神经肽。这两个Carabus物种的成熟神经肽的序列高度相似,其祖先相距约13 Mya,在单拷贝神经肽中未发现序列取代。代表迄今为止从鞘翅目中发现的最多的神经肽。这两个Carabus物种的成熟神经肽的序列高度相似,其祖先相距约13 Mya,在单拷贝神经肽中未发现序列取代。代表迄今为止从鞘翅目中发现的最多的神经肽。这两个Carabus物种的成熟神经肽的序列高度相似,其祖先相距约13 Mya,在单拷贝神经肽中未发现序列取代。
更新日期:2019-12-21
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