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Exploring the phytochemical profile of Cytinus hypocistis (L.) L. as a source of health-promoting biomolecules behind its in vitro bioactive and enzyme inhibitory properties.
Food and Chemical Toxicology ( IF 4.3 ) Pub Date : 2019-12-21 , DOI: 10.1016/j.fct.2019.111071
Ana Rita Silva 1 , José Pinela 2 , Maria Inês Dias 2 , Ricardo C Calhelha 2 , Maria José Alves 2 , Andrei Mocan 3 , Pablo A García 4 , Lillian Barros 2 , Isabel C F R Ferreira 2
Affiliation  

Cytinus hypocistis whole plant and its three different parts (petals, stalks, and nectar) were chemically characterised and their biological properties evaluated. A total of 17 phenolic compounds were identified, being galloyl-bis-HHDP-glucose the most abundant. All the tested extracts showed high antioxidant capacity, with the petals exhibiting the most promising results both in the OxHLIA (IC50 = 0.279 ng/mL) and TBARS (IC50 = 0.342 ng/mL) assays. For the antidiabetic and anti-tyrosinase enzyme inhibitory assays, the stalk extract presented the lowest IC50 values, 0.039 mg/mL and 0.09 mg/mL, respectively. Regarding antibacterial activity, all tested extracts displayed broad-spectrum microbial inhibition against both Gram-positive and Gram-negative bacteria. Similarly, all extracts displayed effective anti-proliferation activity against four tested tumour cell lines (NCI-H460, HeLa, HepG2, and MCF-7), with no toxicity observed for a non-tumour cell line. Considering the anti-inflammatory activity, the petals showed the highest nitric oxide inhibition (IC50 = 127 μg/mL). These results point C. hypocistis as a promising source of health-promoting biomolecules.

中文翻译:

探索Cytinus hypocistis(L.)L.的植物化学特征,因为它具有体外生物活性和酶抑制特性,可作为促进健康的生物分子的来源。

对Cycyus hypocistis整株植物及其三个不同部分(花瓣,茎和花蜜)进行了化学表征,并对其生物学特性进行了评估。总共鉴定出17种酚类化合物,其中最丰富的是没食子酰基-双-HHDP-葡萄糖。所有测试的提取物均显示出高抗氧化能力,在OxHLIA(IC50 = 0.279 ng / mL)和TBARS(IC50 = 0.342 ng / mL)分析中,花瓣表现出最有希望的结果。对于抗糖尿病和抗酪氨酸酶抑制试验,茎提取物的IC50值最低,分别为0.039 mg / mL和0.09 mg / mL。关于抗菌活性,所有测试的提取物均表现出对革兰氏阳性和革兰氏阴性细菌的广谱微生物抑制作用。相似地,所有提取物均对四种测试的肿瘤细胞系(NCI-H460,HeLa,HepG2和MCF-7)显示出有效的抗增殖活性,未观察到非肿瘤细胞系的毒性。考虑到抗炎活性,花瓣对一氧化氮的抑制作用最高(IC50 = 127μg/ mL)。这些结果表明,C.hypercistis是促进健康的生物分子的有希望的来源。
更新日期:2019-12-21
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