AtMOB1 Genes Regulate Jasmonate Accumulation and Plant Development.,Plant Physiology

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AtMOB1 Genes Regulate Jasmonate Accumulation and Plant Development.
Plant Physiology ( IF 6.5 ) Pub Date : 2019-12-20 , DOI: 10.1104/pp.19.01434
Zhiai Guo ₁ , Xiaozhen Yue _{1,

2} , Xiaona Cui _{1,

2} , Lizhen Song ₁ , Youfa Cheng _{2,

3,

4}

Affiliation

The MOB1 proteins are highly conserved in yeasts, animals, and plants. Previously, we showed that the Arabidopsis (Arabidopsis thaliana) MOB1A gene (AtMOB1A/NCP1) plays critical roles in auxin-mediated plant development. Here, we report that AtMOB1A and AtMOB1B redundantly and negatively regulate jasmonate (JA) accumulation and function in Arabidopsis development. The two MOB1 genes exhibited similar expression patterns, and the MOB1 proteins displayed similar subcellular localizations and physically interacted in vivo. Furthermore, the atmob1a atmob1b (mob1a/1b) double mutant displayed severe developmental defects, which were much stronger than those of either single mutant. Interestingly, many JA-related genes were up-regulated in mob1a/1b, suggesting that AtMOB1A and AtMOB1B negatively regulate the JA pathways. mob1a/1b plants accumulated more JA and were hypersensitive to exogenous JA treatments. Disruption of MYC2, a key gene in JA signaling, in the mob1a/1b background partially alleviated the root defects and JA hypersensitivity observed in mob1a/1b. Moreover, the expression levels of the MYC2-repressed genes PLT1 and PLT2 were significantly decreased in the mob1a/1b double mutant. Our results showed that MOB1A/1B genetically interact with SIK1 and antagonistically modulate JA-related gene expression. Taken together, our findings indicate that AtMOB1A and AtMOB1B play important roles in regulating JA accumulation and Arabidopsis development.

中文翻译：

AtMOB1基因调节茉莉酸酯的积累和植物发育。

MOB1蛋白在酵母，动物和植物中高度保守。以前，我们表明拟南芥（Arabidopsis thaliana）MOB1A基因（AtMOB1A / NCP1）在生长素介导的植物发育中起关键作用。在这里，我们报告AtMOB1A和AtMOB1B冗余和负调控茉莉（JA）积累和在拟南芥发育中的功能。这两个MOB1基因表现出相似的表达模式，并且MOB1蛋白表现出相似的亚细胞定位并在体内发生物理相互作用。此外，atmob1a atmob1b（mob1a / 1b）双突变体显示出严重的发育缺陷，比任何一个单突变体都强得多。有趣的是，mob1a / 1b中许多与JA相关的基因被上调，这表明AtMOB1A和AtMOB1B对JA通路起负调控作用。mob1a / 1b植物积累了更多的JA，并且对外源JA处理非常敏感。在mob1a / 1b背景中，JA信号中的关键基因MYC2的破坏部分缓解了mob1a / 1b中观察到的根部缺损和JA超敏反应。此外，在mob1a / 1b双重突变体中，MYC2抑制基因PLT1和PLT2的表达水平显着降低。我们的结果表明，MOB1A / 1B与SIK1发生遗传相互作用并拮抗JA相关基因的表达。综上所述，我们的发现表明AtMOB1A和AtMOB1B在调节JA积累和拟南芥发育中起重要作用。此外，在mob1a / 1b双重突变体中，MYC2抑制基因PLT1和PLT2的表达水平显着降低。我们的结果表明，MOB1A / 1B与SIK1发生遗传相互作用并拮抗JA相关基因的表达。综上所述，我们的发现表明AtMOB1A和AtMOB1B在调节JA积累和拟南芥发育中起重要作用。此外，在mob1a / 1b双重突变体中，MYC2抑制基因PLT1和PLT2的表达水平显着降低。我们的结果表明，MOB1A / 1B与SIK1发生遗传相互作用并拮抗JA相关基因的表达。综上所述，我们的发现表明AtMOB1A和AtMOB1B在调节JA积累和拟南芥发育中起重要作用。

更新日期：2020-03-03

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