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Ureide Permease 5 (AtUPS5) Connects Cell Compartments Involved in Ureide Metabolism.
Plant Physiology ( IF 6.5 ) Pub Date : 2019-12-20 , DOI: 10.1104/pp.19.01136 Ignacio Lescano 1 , María Florencia Bogino 2 , Carolina Martini 1, 2 , Tomás María Tessi 1 , Claudio Alejandro González 1, 2 , Karin Schumacher 3 , Marcelo Desimone 2, 4
Plant Physiology ( IF 6.5 ) Pub Date : 2019-12-20 , DOI: 10.1104/pp.19.01136 Ignacio Lescano 1 , María Florencia Bogino 2 , Carolina Martini 1, 2 , Tomás María Tessi 1 , Claudio Alejandro González 1, 2 , Karin Schumacher 3 , Marcelo Desimone 2, 4
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Allantoin is a purine oxidative product involved in long distance transport of organic nitrogen in nodulating legumes and was recently shown to play a role in stress tolerance in other plants. The subcellular localization of enzymes that catalyze allantoin synthesis and degradation indicates that allantoin is produced in peroxisomes and degraded in the endoplasmic reticulum (ER). Although it has been determined that allantoin is mostly synthesized in roots and transported to shoots either for organic nitrogen translocation in legumes or for plant protection during stress in Arabidopsis (Arabidopsis thaliana), the mechanism and molecular components of allantoin export from root cells are still unknown. AtUPS5 (Arabidopsis UREIDE PERMEASE 5) is a transmembrane protein that transports allantoin with high affinity when expressed in yeast. The subcellular fate of splicing variants AtUPS5L (long) and AtUPS5S (short) was studied by tagging them with fluorescent proteins in their cytosolic loops. The capability of these fusion proteins to complement the function of the native proteins was demonstrated by nutritional and salt stress experiments. Both variants localized to the ER, but the AtUPS5L variant was also detected in the trans-Golgi network/early endosome and at the plasma membrane. AtUPS5L and AtUPS5S localization indicates that they could have different roles in allantoin distribution between subcellular compartments. Our data suggest that under nonstress conditions UPS5L and UPS5S may function in allantoin degradation for nutrient recycling, whereas under stress, both genes may be involved in vesicular export allowing allantoin translocation from roots to shoots.
中文翻译：
Ureide Permease 5 (AtUPS5) 连接参与 Ureide 代谢的细胞室。
尿囊素是一种嘌呤氧化产物，参与结瘤豆科植物中有机氮的长距离运输，最近被证明在其他植物的胁迫耐受性中发挥作用。催化尿囊素合成和降解的酶的亚细胞定位表明尿囊素是在过氧化物酶体中产生并在内质网（ER）中降解。尽管已经确定尿囊素主要在根部合成并转运至地上部用于豆科植物中的有机氮转运或拟南芥（Arabidopsis thaliana）胁迫期间的植物保护，但尿囊素从根细胞输出的机制和分子成分仍不清楚。 AtUPS5（拟南芥 UREIDE PERMEASE 5）是一种跨膜蛋白，当在酵母中表达时，它能以高亲和力转运尿囊素。通过在细胞质环中用荧光蛋白标记剪接变体 AtUPS5L（长）和 AtUPS5S（短），研究了它们的亚细胞命运。营养和盐胁迫实验证明了这些融合蛋白补充天然蛋白功能的能力。两种变体都定位于内质网，但在反高尔基体网络/早期内体和质膜中也检测到了 AtUPS5L 变体。 AtUPS5L 和 AtUPS5S 定位表明它们在亚细胞区室之间的尿囊素分布中可能具有不同的作用。我们的数据表明，在非胁迫条件下，UPS5L 和 UPS5S 可能在尿囊素降解中发挥作用，以实现养分循环，而在胁迫下，这两个基因可能参与囊泡输出，使尿囊素从根转移到芽。
更新日期：2020-03-03
中文翻译：
Ureide Permease 5 (AtUPS5) 连接参与 Ureide 代谢的细胞室。
尿囊素是一种嘌呤氧化产物，参与结瘤豆科植物中有机氮的长距离运输，最近被证明在其他植物的胁迫耐受性中发挥作用。催化尿囊素合成和降解的酶的亚细胞定位表明尿囊素是在过氧化物酶体中产生并在内质网（ER）中降解。尽管已经确定尿囊素主要在根部合成并转运至地上部用于豆科植物中的有机氮转运或拟南芥（Arabidopsis thaliana）胁迫期间的植物保护，但尿囊素从根细胞输出的机制和分子成分仍不清楚。 AtUPS5（拟南芥 UREIDE PERMEASE 5）是一种跨膜蛋白，当在酵母中表达时，它能以高亲和力转运尿囊素。通过在细胞质环中用荧光蛋白标记剪接变体 AtUPS5L（长）和 AtUPS5S（短），研究了它们的亚细胞命运。营养和盐胁迫实验证明了这些融合蛋白补充天然蛋白功能的能力。两种变体都定位于内质网，但在反高尔基体网络/早期内体和质膜中也检测到了 AtUPS5L 变体。 AtUPS5L 和 AtUPS5S 定位表明它们在亚细胞区室之间的尿囊素分布中可能具有不同的作用。我们的数据表明，在非胁迫条件下，UPS5L 和 UPS5S 可能在尿囊素降解中发挥作用，以实现养分循环，而在胁迫下，这两个基因可能参与囊泡输出，使尿囊素从根转移到芽。
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