Here we report an eco-friendly and robust silver nanoparticles (AgNPs) as a simple and sensitive probe for the detection of melamine in biological samples. The AgNPs were synthesized using the extract of Colocasia esculenta. The spherical shaped and monodispersed AgNPs were obtained with an average particle size of 20 nm. The as synthesized colorimetric probe was morphologically and spectroscopically characterized using transmission electron microscope and fourier-transform infrared and UV–vis spectrometry respectively. This method is based on the melamine induced aggregation of AgNPs, hence, color changes from yellow to orange red. Various experimental parameters such as effect of pH and time on the detection of melamine was investigated to enhance the sensitivity of the developed method. Under optimized conditions, the limit of detection and limit of quantification were found to be 0.070 ppm and 1.60 ppm respectively with a recovery values ranging from 95.0–103%. The findings of this study resulted in the development of highly sensitive and selective sensing probe for the detection of melamine in biological samples.

在这里，我们报告了一种生态友好且坚固耐用的银纳米颗粒（AgNPs），作为检测生物样品中三聚氰胺的简单而灵敏的探针。AgCos是用Colocasia esculenta提取物合成的。获得了球形且单分散的AgNP，其平均粒径为20 nm。分别使用透射电子显微镜和傅立叶变换红外光谱和紫外可见光谱对形态学和光谱学特征进行了表征。该方法基于三聚氰胺诱导的AgNP聚集，因此颜色从黄色变为橘红色。研究了各种实验参数，例如pH和时间对三聚氰胺检测的影响，以提高所开发方法的灵敏度。在最佳条件下，检测限和定量限分别为0.070 ppm和1.60 ppm，回收率范围为95.0–103％。