AIM Adverse and advanced prognostic signs in IgA nephropathy (IgAN) are interstitial fibrosis and tubular atrophy, but early predictors of bad outcome are still lacking. We investigated expression of connective tissue growth factor (CTGF) and c-Myb in renal biopsies of IgAN and Henoch-Schönlein purpura (HSP), because these gene products are indirectly included in fibrosis and epithelial-mesenchymal transition (EMT). METHODS The sample included 23 patients and 8 controls who underwent nephrectomy due to renal cancer. The slides cut from the paraffin blocks were prepared for standard indirect immunoflourescence, using antibodies to CTGF and c-Myb. Ten high-power non-overlapping fields were photographed on Olympus IX51 microscope. Average percent of positive tubular cells, as well as number of positive cells per glomerulus were calculated. RESULTS The cytoplasmic tubular CTGF expression was higher in IgAN/HSP than in controls (P < 0.001), whereas no difference was found in glomeruli (P = 0.437). The nuclear c-Myb expresssion in glomeruli and tubules was higher in IgAN/HSP than in controls (P < 0.05). In the follow-up, decline in renal function correlated with glomerular and tubular c-Myb, as well as tubular CTGF expression (all P < 0.05). CONCLUSION Our results proposed c-Myb and CTGF as novel, early and sensitive markers of chronic kidney disease and worse renal outcome, but larger series are needed.

中文翻译：

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结缔组织生长因子和c-Myb表达在IgA肾病和Henoch-Schönlein紫癜-A免疫组化研究中的预后价值。

目的IgA肾病（IgAN）的不良和晚期预后体征是间质纤维化和肾小管萎缩，但仍缺乏不良预后的早期预测指标。我们研究了结缔组织生长因子（CTGF）和c-Myb在IgAN和Henoch-Schönlein紫癜（HSP）的肾活检中的表达，因为这些基因产物间接包括在纤维化和上皮-间质转化（EMT）中。方法样本包括23例因肾癌行肾切除术的患者和8例对照。使用针对CTGF和c-Myb的抗体，将制备自石蜡块的玻片用于标准间接免疫荧光。在Olympus IX51显微镜上拍摄了十个大功率非重叠场。计算阳性肾小管细胞的平均百分比，以及每肾小球的阳性细胞数。结果IgAN / HSP中胞浆小管CTGF的表达高于对照组（P <0.001），而肾小球未见差异（P = 0.437）。IgAN / HSP中肾小球和肾小管的核c-Myb表达高于对照组（P <0.05）。在随访中，肾功能下降与肾小球和肾小管c-Myb以及肾小管CTGF表达相关（所有P <0.05）。结论我们的研究结果提示c-Myb和CTGF是慢性肾脏病和较差的肾脏结局的新颖，早期和敏感的标志物，但还需要更大的研究范围。在随访中，肾功能下降与肾小球和肾小管c-Myb以及肾小管CTGF表达相关（所有P <0.05）。结论我们的研究结果提示c-Myb和CTGF是慢性肾脏病和较差的肾脏结局的新颖，早期和敏感的标志物，但还需要更大的研究范围。在随访中，肾功能下降与肾小球和肾小管c-Myb以及肾小管CTGF表达相关（所有P <0.05）。结论我们的研究结果提示c-Myb和CTGF是慢性肾脏疾病和较差的肾脏结局的新颖，早期和敏感的标志物，但还需要更大的研究范围。