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Identification of a 7-microRNA signature in plasma as promising biomarker for nasopharyngeal carcinoma detection.
Cancer Medicine ( IF 2.9 ) Pub Date : 2019-12-19 , DOI: 10.1002/cam4.2676 Huo Zhang 1, 2 , Xuan Zou 3 , Lirong Wu 4 , Shiyu Zhang 1 , Tongshan Wang 1 , Ping Liu 1 , Wei Zhu 1 , Jun Zhu 4
Cancer Medicine ( IF 2.9 ) Pub Date : 2019-12-19 , DOI: 10.1002/cam4.2676 Huo Zhang 1, 2 , Xuan Zou 3 , Lirong Wu 4 , Shiyu Zhang 1 , Tongshan Wang 1 , Ping Liu 1 , Wei Zhu 1 , Jun Zhu 4
Affiliation
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BACKGROUND
Circulating microRNAs (miRNAs) have become reliable sources of non-invasive biomarkers for cancer diagnosis. Identification of promising miRNA biomarkers in plasma might benefit a lot to the detection of nasopharyngeal carcinoma (NPC).
METHODS
The Exiqon miRNA qPCR panel was used in the screening stage to identify candidate miRNAs, which were further verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the following three stages among plasma samples from 200 NPC patients and 189 healthy donors (as normal controls [NCs]). The identified miRNAs were further explored in tissue specimens (48 NPC vs 32 NCs) and plasma exosomes (32 NPC vs 32 NCs). Survival analyses were ultimately conducted by Cox regression models and Kaplan-Meier curves using log-rank tests.
RESULTS
We identified a 7-miRNA signature including let-7b-5p, miR-140-3p, miR-144-3p, miR-17-5p, miR-20a-5p, miR-20b-5p, and miR-205-5p in plasma for NPC diagnosis after four-stage validation. The areas under the receiver operating characteristic curve (AUCs) for the signature were 0.879, 0.884, 0.921, and 0.807 for the training, testing, external validation stage, and the combined three stages, respectively. In NPC tissues, miR-144-3p, miR-17-5p, miR-20a-5p, and miR-205-5p were consistently up-regulated while let-7b-5p and miR-140-3p were significantly down-regulated compared to NCs. However, none of the seven identified miRNAs were dysregulated in plasma-derived exosomes in NPC patients. As to survival analysis, none of the seven miRNAs seemed to be associated with NPC prognosis.
CONCLUSION
We identified a 7-miRNA signature in plasma as promising non-invasive biomarkers for NPC detection.
中文翻译：
在血浆中鉴定7-microRNA标记物作为鼻咽癌检测的有前途的生物标志物。
背景技术循环微RNA（miRNA）已经成为用于癌症诊断的非侵入性生物标志物的可靠来源。血浆中有希望的miRNA生物标志物的鉴定可能对检测鼻咽癌（NPC）很有帮助。方法在Exiqon miRNA qPCR面板的筛选阶段鉴定候选miRNA，并在以下三个阶段对来自200名NPC患者和189名健康供体的血浆样品进行定量逆转录聚合酶链反应（qRT-PCR）进一步验证了这些候选miRNA（作为常规控件[NC]）。在组织标本（48 NPC vs 32 NCs）和血浆外泌体（32 NPC vs 32 NCs）中进一步探索鉴定出的miRNA。生存分析最终通过对数秩检验通过Cox回归模型和Kaplan-Meier曲线进行。结果我们确定了7-miRNA标记，包括let-7b-5p，miR-140-3p，miR-144-3p，miR-17-5p，miR-20a-5p，miR-20b-5p和miR-205-经过四阶段验证后，血浆中的5p可用于NPC诊断。在训练，测试，外部验证阶段和合并的三个阶段中，签名的接收器工作特性曲线（AUC）下的面积分别为0.879、0.884、0.921和0.807。在NPC组织中，miR-144-3p，miR-17-5p，miR-20a-5p和miR-205-5p始终被上调，而let-7b-5p和miR-140-3p被显着下调。与NCs相比。但是，在NPC患者的血浆来源的外泌体中，七个已鉴定的miRNA均未失调。至于生存分析，这七个miRNA似乎都与NPC的预后无关。
更新日期：2019-12-20
中文翻译：
在血浆中鉴定7-microRNA标记物作为鼻咽癌检测的有前途的生物标志物。
背景技术循环微RNA（miRNA）已经成为用于癌症诊断的非侵入性生物标志物的可靠来源。血浆中有希望的miRNA生物标志物的鉴定可能对检测鼻咽癌（NPC）很有帮助。方法在Exiqon miRNA qPCR面板的筛选阶段鉴定候选miRNA，并在以下三个阶段对来自200名NPC患者和189名健康供体的血浆样品进行定量逆转录聚合酶链反应（qRT-PCR）进一步验证了这些候选miRNA（作为常规控件[NC]）。在组织标本（48 NPC vs 32 NCs）和血浆外泌体（32 NPC vs 32 NCs）中进一步探索鉴定出的miRNA。生存分析最终通过对数秩检验通过Cox回归模型和Kaplan-Meier曲线进行。结果我们确定了7-miRNA标记，包括let-7b-5p，miR-140-3p，miR-144-3p，miR-17-5p，miR-20a-5p，miR-20b-5p和miR-205-经过四阶段验证后，血浆中的5p可用于NPC诊断。在训练，测试，外部验证阶段和合并的三个阶段中，签名的接收器工作特性曲线（AUC）下的面积分别为0.879、0.884、0.921和0.807。在NPC组织中，miR-144-3p，miR-17-5p，miR-20a-5p和miR-205-5p始终被上调，而let-7b-5p和miR-140-3p被显着下调。与NCs相比。但是，在NPC患者的血浆来源的外泌体中，七个已鉴定的miRNA均未失调。至于生存分析，这七个miRNA似乎都与NPC的预后无关。
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