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Physico-chemical behaviors of human and bovine milk membrane extracts and their influence on gastric lipase adsorption.
Biochimie ( IF 3.3 ) Pub Date : 2019-12-20 , DOI: 10.1016/j.biochi.2019.12.003
Claire Bourlieu 1 , Wafa Mahdoueni 2 , Gilles Paboeuf 3 , Eric Gicquel 4 , Olivia Ménard 2 , Stéphane Pezennec 2 , Said Bouhallab 2 , Amélie Deglaire 2 , Didier Dupont 2 , Frédéric Carrière 5 , Véronique Vié 3
Affiliation  

Milk fat globule membrane conditions the reactivity and enzymatic susceptibility of milk lipids. The use of bovine membrane extracts to make infant formulas more biomimetic of human milk has been suggested recently. A comparison of the physico-chemical behavior of human and bovine milk membrane extracts and their interaction with gastric lipase is here undertaken using biophysical tools. Milk membrane extracts (70% of polar lipids) were obtained either pooling of mature human milk (n = 5) or bovine buttermilk. Human extract contained more anionic glycerophospholipids, less phosphatidylethanolamine and more unsaturated fatty acids (57% versus 46%) than bovine extract. Human extract presented a higher compressibility, with slower increase of surface pressure, than bovine extract. Micronic liquid condensed (LC) domains were evidenced in both extracts at 10 mN/m, but the evolution differs upon compression. Upon gastric lipase addition, an adsorption preference for liquid expanded phase (LE) was observed for both extracts. However, insertion was more homogeneous in terms of height level in human extract and impacted less its lipid lateral organization than in bovine extract. Both membrane extracts share close physico-chemical properties, however human membrane higher compressibility may favour gastric lipase insertion and higher interfacial reactivity in gastric conditions.

中文翻译:

人和牛乳膜提取物的理化行为及其对胃脂肪酶吸附的影响。

乳脂小球膜调节乳脂的反应性和酶敏感性。最近,有人建议使用牛膜提取物使婴儿配方奶粉更像人乳。使用生物物理工具比较人乳和牛乳膜提取物的理化行为及其与胃脂肪酶的相互作用。收集成熟的人乳(n = 5)或牛酪乳获得乳膜提取物(极性脂质的70%)。人提取物比牛提取物含有更多的阴离子甘油磷脂,更少的磷脂酰乙醇胺和更多的不饱和脂肪酸(57%比46%)。与牛提取物相比,人提取物具有更高的可压缩性,且表面压力的升高速度较慢。在两种提取物中均以10 mN / m的速度证实了微子液体冷凝(LC)域,但压缩后的演化有所不同。加入胃脂肪酶后,两种提取物均观察到对液体膨胀相(LE)的吸附偏好。然而,就人提取物的高度水平而言,插入更为均匀,并且其脂类侧向组织的影响比牛提取物中的影响小。两种膜提取物都具有接近的理化性质,但是人膜更高的可压缩性可能有利于胃脂肪酶的插入和胃部条件下更高的界面反应性。在人类提取物中,插入在高度水平上更为均一,并且其脂类侧向组织的影响比在牛提取物中的影响小。两种膜提取物都具有接近的理化性质,但是人膜更高的可压缩性可能有利于胃脂肪酶的插入和胃部条件下更高的界面反应性。在人类提取物中,插入在高度水平上更为均一,并且其脂类侧向组织的影响比在牛提取物中的影响小。两种膜提取物都具有接近的理化性质,但是人膜更高的可压缩性可能有利于胃脂肪酶的插入和胃部条件下更高的界面反应性。
更新日期:2019-12-20
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