This study evaluates the impact of two plant sterol (PS) enriched fruit beverages (0.6 g /100 ml), without (MfB) or with GOS (MfB-G) (1.2 g/100 ml) on the resistance against oxidative stress induced by non-conventional (cholesterol oxidation products (COPs)) and conventional (H₂O₂) oxidant compounds, as well as their impact on C. elegans longevity. Nematodes fed with both beverages (0.005–0.25%, v/v) showed similar improved oxidative stress resistance against COPs and H₂O₂. This effect was dependent on daf-16 transcription factor, although GOS showed an additional beneficial effect independent to daf-16. In addition, both beverages extended nematode lifespan, independently of the presence of GOS. Longevity assays using daf-16 and daf-2 mutant strains revealed that the observed effect was potentially linked to the insulin-IGF-1 pathway. These results provide new in vivo evidence for the potential use of PS enriched fruit beverages in the prevention of oxidative stress underlying many diseases.

这项研究评估了两种富含植物固醇（PS）的水果饮料（0.6 g / 100 ml），不含（MfB）或含有GOS（MfB-G）（1.2 g / 100 ml）的饮料对由氧化应激引起的抗氧化性的影响非常规（胆固醇氧化产物（COPs））和常规（H ₂ O ₂）氧化剂化合物，以及它们对秀丽隐杆线虫寿命的影响。两种饮料喂养的线虫（0.005-0.25％，v / v）对COPs和H ₂ O _2的氧化应激表现出相似的改善。尽管GOS显示了独立于daf-16的其他有益作用，但该作用取决于daf-16转录因子。此外，两种饮料都可以延长线虫的寿命，而与GOS的存在无关。使用daf-16和daf-2突变菌株进行的寿命试验表明，观察到的作用可能与胰岛素-IGF-1途径有关。这些结果为富含PS的果汁饮料在预防许多疾病的氧化应激中的潜在用途提供了新的体内证据。