As a typical green methodology, enzymatic catalysis has been extensively employed in multitudinous chemical and biological transformation procedures. However, intrinsic fragile nature of enzymes makes them prone to denaturation or destabilization in harsh practical conditions, leading to unavoidably shortened lifespan and extremely high cost. It was proven that enzyme immobilization is an efficient strategy for enhancing their catalytic performance in continuous industrial practices. Metal-Organic Frameworks (MOFs) with extremely high specific surface area, abundant porosity, extraordinary multifunctionality, and relatively high stability, in recent years, have attracted remarkable research interests as novel supporting matrices for efficient enzyme immobilization and protection. Many reported MOFs-enzyme composites exhibit unprecedented catalytic performances than those of free enzymes, including improved enzyme efficiency, stability, selectivity, and recyclability, due to the protection of enzymes by highly ordered frameworks. To present a systematic overview of this emerging and developing field, herein, we summarize an update review about the most recent advances in MOFs immobilizing enzymes from the aspects of general synthetic approaches, critical impact factors, enhanced catalytic performances, and the practical applications. Subsequently, the emerging theories, methodologies and technologies in this thriving area are briefly introduced. Finally, barriers and future perspectives about MOFs for enzyme immobilization are also discussed.

作为一种典型的绿色方法，酶催化已广泛用于多种化学和生物转化程序中。然而，酶固有的易碎性质使它们在苛刻的实际条件下易于变性或不稳定，导致不可避免地缩短了寿命并极大地提高了成本。事实证明，酶的固定化是在连续工业实践中提高其催化性能的有效策略。近年来，具有极高的比表面积，丰富的孔隙率，非凡的多功能性和相对较高的稳定性的金属有机骨架（MOF）作为有效支持酶固定和保护的新型支撑基质已引起了广泛的研究兴趣。许多报道的MOF-酶复合物比游离酶具有空前的催化性能，包括酶效率，稳定性，选择性和可回收性的提高，这归因于酶受到高度有序框架的保护。为了在此新兴领域的系统化概述，我们从一般合成方法，关键影响因素，增强的催化性能和实际应用等方面，总结了有关MOF固定化酶的最新进展的最新综述。随后，简要介绍了这一蓬勃发展领域中的新兴理论，方法和技术。最后，还讨论了用于酶固定化的MOF的障碍和未来展望。稳定性，选择性和可回收性，这归因于高度有序框架对酶的保护。为了在此新兴领域的系统化概述，我们从一般合成方法，关键影响因素，增强的催化性能和实际应用等方面，总结了有关MOF固定化酶的最新进展的最新综述。随后，简要介绍了这一蓬勃发展领域中出现的理论，方法和技术。最后，还讨论了用于酶固定化的MOF的障碍和未来展望。稳定性，选择性和可回收性，这归因于高度有序框架对酶的保护。为了在此新兴领域的系统化概述，我们从一般合成方法，关键影响因素，增强的催化性能和实际应用等方面，总结了有关MOF固定化酶的最新进展的最新综述。随后，简要介绍了这一蓬勃发展领域中的新兴理论，方法和技术。最后，还讨论了用于酶固定化的MOF的障碍和未来展望。我们从一般合成方法，关键影响因素，增强的催化性能和实际应用等方面总结了有关MOF固定化酶最新进展的最新综述。随后，简要介绍了这一蓬勃发展领域中出现的理论，方法和技术。最后，还讨论了用于酶固定化的MOF的障碍和未来展望。我们从一般合成方法，关键影响因素，增强的催化性能和实际应用等方面总结了有关MOF固定化酶最新进展的最新综述。随后，简要介绍了这一蓬勃发展领域中的新兴理论，方法和技术。最后，还讨论了用于酶固定化的MOF的障碍和未来展望。