Abstract Extraintestinal pathogenic Escherichia coli (ExPEC) are responsible for urinary tract infections, inflammatory bowel disease, sepsis and meningitis. Retail poultry meat has been identified as the main reservoir for ExPEC in food. Information regarding ExPEC virulence factor (VF) or antibiotic resistance (AR) involvement with resistance to high-pressure inactivation in food is lacking. In this study we inoculated ground chicken meat with 22 individual isolates of clinical uropathogenic E. coli (UPEC) and newborn meningitis causing E. coli (NMEC), and isolates from retail chicken meat. We then determined their high-pressure inactivation kinetics (D10-value). The mean D10-value for all isolates (n = 22) was 3.26 min at 400 MPa. The mean D10-value for the UPEC, NMEC, chicken isolates were 5.0, 1.3, 2.23 and 4.98 min at 400 MPa, respectively. The D10 varied widely between the 22 isolates. The mean D10-value for the clinical isolates was 3.33 vs. 3.15 for the non-clinical isolates. The NMEC strains were more sensitive to HPP. ExPEC lacking chuA, cnf1, sinH, papG, hlyA, vat, yncD were more resistant to HPP, indicating ExPEC VF could play a role in high pressure resistance. HPP at 600 MPa for 3 min resulted in >6 log10 reductions for UPEC, NMEC, and retail chicken product isolates.

中文翻译：

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高压处理灭活鸡绞肉中悬浮的肠外病原性大肠杆菌及影响抗高压能力的毒力因子的鉴定

摘要 肠外致病性大肠杆菌 (ExPEC) 是导致尿路感染、炎症性肠病、败血症和脑膜炎的原因。零售禽肉已被确定为食品中 ExPEC 的主要储存库。缺乏有关 ExPEC 毒力因子 (VF) 或抗生素抗性 (AR) 与食品中高压灭活抗性相关的信息。在这项研究中，我们用 22 种单独的临床尿路致病性大肠杆菌 (UPEC) 和引起新生儿脑膜炎的大肠杆菌 (NMEC) 和零售鸡肉的分离物接种碎鸡肉。然后我们确定了它们的高压灭活动力学（D10 值）。所有分离株 (n = 22) 的平均 D10 值在 400 MPa 下为 3.26 分钟。UPEC、NMEC、鸡分离物的平均 D10 值在 400 MPa 下分别为 5.0、1.3、2.23 和 4.98 分钟。D10 在 22 个分离株之间变化很大。临床分离株的平均 D10 值为 3.33，而非临床分离株为 3.15。NMEC 菌株对 HPP 更敏感。缺少chuA、cnf1、sinH、papG、hlyA、vat、yncD的ExPEC对HPP的抵抗力更强，表明ExPEC VF可以发挥抗高压作用。HPP 在 600 MPa 下持续 3 分钟导致 UPEC、NMEC 和零售鸡肉产品分离物的 log10 减少 >6。