Context: Vitis vinifera leaves are traditionally used in Tunisian folk medicine to treat digestive pathologies.Objective: We aimed to compare the gastroprotective effects of hydromethanolic leaves extracts of wild and cultivated grapes accessions native of Tunisia.Materials and methods: The phytochemical analysis of grapevine leaves extracts was performed. The gastroprotective activity was evaluated by ethanol-induced gastric-ulcer in rats pre-treated with increased doses of the extracts or with the standard omeprazole. Index of gastric secretions (volume, pH and gastric mucus production), stomach wall histology and biochemical parameters were estimated for assessment of anti-secretory and gastroprotective effects of the extracts.Results: Pre-treatment with grapevine leaves extracts decreased significantly gastric volume, gastric mucosal damage and increased significantly gastric juice pH compared with the negative control group. The extracts prevented ethanol-induced decrease of the activity of antioxidant enzymes while the levels of malondialdehyde and of reduced glutathione were decreased significantly. Moreover, the most marked effect was observed at low doses of wild ecotype 'Nefza-I' extracts.Conclusion: The leaves of Vitis species might be suitable as a functional food for therapeutic purpose and demonstrates gastroprotective action in gastric lesions model. Both accessions exhibited gastroprotective effects, but wild 'Nefza-I' ecotype was more effective than cultivar 'Marsaoui'.

中文翻译：

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在突尼斯北部种植的两个野生葡萄和葡萄品种的叶提取物对大鼠乙醇诱导的氧化应激的胃保护作用。

背景：葡萄叶在突尼斯民间医学中通常用于治疗消化道疾病。目的：我们旨在比较突尼斯天然和栽培葡萄种的氢甲醇叶提取物的胃保护作用。材料和方法：葡萄叶的植物化学分析进行提取。通过乙醇诱导的胃溃疡评估大鼠的胃保护活性，所述大鼠用增加剂量的提取物或标准奥美拉唑进行了预处理。评估胃分泌物的指数（量，pH和胃粘液产生），胃壁组织学和生化参数，以评估提取物的抗分泌和胃保护作用。结果：葡萄叶提取物的预处理显着降低了胃体积，与阴性对照组相比，胃粘膜损害和胃液pH值显着增加。提取物阻止了乙醇诱导的抗氧化酶活性的降低，而丙二醛和还原型谷胱甘肽的水平则显着降低。此外，在低剂量的野生生态型“ Nefza-I”提取物中观察到最显着的效果。结论：葡萄树种的叶子可能适合作为治疗性功能食品，并在胃部病变模型中显示出胃保护作用。两种种均显示出胃保护作用，但是野生的'Nefza-I'生态型比'Marsaoui'品种更有效。提取物阻止了乙醇诱导的抗氧化酶活性的降低，而丙二醛和还原型谷胱甘肽的水平则显着降低。此外，在低剂量的野生生态型“ Nefza-I”提取物中观察到最显着的效果。结论：葡萄树种的叶子可能适合作为治疗性功能食品，并在胃部病变模型中显示出胃保护作用。两种种均显示出胃保护作用，但是野生的'Nefza-I'生态型比'Marsaoui'品种更有效。提取物阻止了乙醇诱导的抗氧化酶活性的降低，而丙二醛和还原型谷胱甘肽的水平则显着降低。此外，在低剂量的野生生态型“ Nefza-I”提取物中观察到最显着的效果。结论：葡萄树种的叶子可能适合作为治疗性功能食品，并在胃部病变模型中显示出胃保护作用。两种种均显示出胃保护作用，但是野生的'Nefza-I'生态型比'Marsaoui'品种更有效。葡萄树种的叶子可能适合作为功能性食品用于治疗目的，并在胃部病变模型中显示出胃保护作用。两种种均显示出胃保护作用，但是野生的'Nefza-I'生态型比'Marsaoui'品种更有效。葡萄树种的叶子可能适合作为功能性食品用于治疗目的，并在胃部病变模型中显示出胃保护作用。两种种均显示出胃保护作用，但是野生的'Nefza-I'生态型比'Marsaoui'品种更有效。