The separation and purification techniques of chelates can improve the accuracy of detecting results of the chelation rate. As a quantitative indicator of metal ion chelates, the chelation rate can not only reflect the completion of chelation but also determine the amount of metal ions in different forms. The determination of chelation rate can help to determine the suitable chelating reaction conditions, make theoretical basis for the fertilizer efficiency, analyze the stability of chelating fertilizers and study the action mechanism of trace elements. In our study, the methods of separation free metal ions from mixture were reviewed first, including gel filtration chromatography, organic solvent precipitation, ion exchange chromatography, membrane separation and high performance liquid chromatography. Then, the qualitative analysis methods of chelates were introduced briefly, including chemical identification, infrared spectroscopy, ultraviolet spectroscopy. A detailed overview of the quantitative determination methods of chelates were also shown, such as ethylenediaminetetraacetic acid titration, chemical titration, atomic absorption spectrometry, inductively coupled plasma atomic emission spectrometry, inductively coupled plasma mass spectrometry, spectrophotometric, chemical modified electrode. In addition, the merits and demerits of chelated rate determination methods of various determination methods were analyzed, and summarized the applicability of various methods, which provided a theoretical basis for optimizing chelating process, characterizing the structure of chelates and analyzing the mechanism of chelating fertilizer. The current methods of measuring chelation rate were also summarized and prospected.

中文翻译：

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螯合物分离检测方法的研究。

螯合物的分离纯化技术可以提高螯合率检测结果的准确性。作为金属离子螯合物的定量指标，螯合速率不仅可以反映螯合的完成，而且可以确定不同形式的金属离子的量。螯合速率的确定可以帮助确定合适的螯合反应条件，为肥料效率提供理论依据，分析螯合肥料的稳定性并研究微量元素的作用机理。在我们的研究中，首先概述了从混合物中分离游离金属离子的方法，包括凝胶过滤色谱法，有机溶剂沉淀，离子交换色谱法，膜分离和高效液相色谱法。然后，简要介绍了螯合物的定性分析方法，包括化学鉴定，红外光谱，紫外光谱。还显示了螯合剂定量测定方法的详细概述，例如乙二胺四乙酸滴定，化学滴定，原子吸收光谱法，电感耦合等离子体原子发射光谱法，电感耦合等离子体质谱法，分光光度法，化学修饰电极。此外，分析了各种测定方法中螯合速率测定方法的优缺点，总结了各种方法的适用性，为优化螯合工艺，表征螯合物的结构以及分析螯合肥料的机理提供了理论依据。