The development of antibody therapeutics relies on animal models that accurately recapitulate disease biology. Syngeneic mouse models are increasingly used with new molecules to capture the biology of complex cancers and disease states, and to provide insight into the role of the immune system. The establishment of syngeneic mouse models requires the ability to generate surrogate mouse counterparts to antibodies designed for humans. In the field of bispecific antibodies, there remains a dearth of technologies available to generate native IgG-like mouse bispecific antibodies. Thus, we engineered a simple co-expression system for one-step purification of intact mouse IgG1 and IgG2a bispecific antibodies from any antibody pair. We demonstrated proof of concept with CD3/CD20 bispecific antibodies, which highlighted both the quality and efficacy of materials generated by this technology.

中文翻译：

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设计和表征用于同基因模型的小鼠IgG1和IgG2a双特异性抗体。

抗体治疗剂的开发依赖于能够准确概括疾病生物学的动物模型。同系小鼠模型越来越多地与新分子一起使用，以捕获复杂的癌症和疾病状态的生物学信息，并提供对免疫系统作用的洞察力。建立同质小鼠模型需要能够产生与为人类设计的抗体的替代小鼠对应物。在双特异性抗体领域，仍然缺乏可用于产生天然IgG样小鼠双特异性抗体的技术。因此，我们设计了一种简单的共表达系统，用于从任何抗体对中一步纯化完整的小鼠IgG1和IgG2a双特异性抗体。我们展示了CD3 / CD20双特异性抗体的概念验证，