1,3-Propanediol (1,3-PDO) has numerous industrial applications in the synthesis of the monomer of the widely used fiber polytrimethylene terephthalate. In this work, the production of 1,3-PDO by Klebsiella pneumoniae is increased by dual-substrate cultivation and fed-batch fermentation. Experimental results indicate that the production of 1,3-PDO can be elevated to 16.09 g/L using a dual substrate ratio (of glucose to crude glycerol) of 1/30 and to 20.73 g/L using an optimized dual-substrate ratio of 1/20. Ultimately, the optimal dual-substrate feeding for a 5 L scale fed-batch fermenter that maximizes 1,3-PDO production (29.69 g/L) is determined. This production yield is better than that reported in most related studies. Eventually, the molecular weight and chemical structure of 1,3-PDO were obtained by FAB-MS, 1H-NMR, and 13C-NMR. Also, in demonstrating the effectiveness of the fermentation strategy in increasing the production and production yield of 1,3-PDO, experimental results indicate that the fermentation of 1,3-PDO is highly promising for commercialization.

中文翻译：

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探索使用肺炎克雷伯菌生产1，3-丙二醇的双底物栽培策略。

1,3-丙二醇（1,3-PDO）在广泛使用的纤维聚对苯二甲酸丙二醇酯的单体的合成中具有许多工业应用。在这项工作中，通过双重底物培养和分批补料发酵，增加了肺炎克雷伯菌的1,3-PDO产量。实验结果表明，使用1/30的双重底物比率（葡萄糖与粗甘油），可以将1,3-PDO的产量提高到16.09 g / L，使用优化的双重底物比率，可以将1,3-PDO的产量提高到20.73 g / L。 1/20。最终，确定了最大化1，3-PDO产量（29.69 g / L）的5 L规模分批发酵罐的最佳双底物进料。该产量高于大多数相关研究报告的产量。最终，通过FAB-MS，1 H-NMR和13 C-NMR获得1，3-PDO的分子量和化学结构。还，