In diabetic nephropathy (DN), intercellular communication is disrupted. Connexins (Cx) have a crucial role in that process. Dietary ratios and supplementation with polyunsaturated fatty acids (PUFAs) can alleviate diabetic complications and cause alterations in Cx levels. Although pannexins (Panx) share similarities with members of the Cx family, their function in diabetic nephropathy has still not been fully determined. We studied the influence of PUFA supplementation on the immunoexpression of Px1 and Cx family members in diabetic kidneys of rats. Four groups of rats in experimental DM1 model were supplemented with different dietary n-6/n-3 ratios; ≈7 in control (C) and diabetic groups (STZ), ≈ 60 in the STZ + N6 group and ≈ 1 (containing 16% EPA and 19% DHA) in the STZ + N3 group. Immunoexpression of Cx40, Cx43, Cx45 and Panx1 was evaluated in the renal tissue of diabetic rats using immunohistochemistry. Diabetes significantly decreased the protein expression of Cx40 and Cx43 and increased Panx1 protein expression in the renal cortex (p < 0.05-p < 0.01). There was a significant impact of diet on Cx and Panx1 immunoexpression. Dietary supplementation with a high n-6/n-3 ratio downregulated the protein expression of Cx45 and Panx1 in diabetic rats (p < 0.05-p < 0.01), while Cx43 immunoexpression was increased in diabetic rats fed with high and low n-6/n-3 ratios (p < 0.01-p < 0.001). Hyperglycaemic conditions in DN interfere with cell-to-cell communication and disturb the connection between cells and their immediate environment due to variations in connexin and pannexin immunoexpression. These variations can be regulated by PUFA dietary intake, suggesting their beneficial effect and possible therapeutic option.

中文翻译：

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PUFAs的添加会影响大鼠糖尿病肾中Pannexin 1和connexins的肾脏表达。

在糖尿病性肾病（DN）中，细胞间通讯受到破坏。连接蛋白（Cx）在该过程中起着至关重要的作用。饮食比例和补充多不饱和脂肪酸（PUFA）可以减轻糖尿病并发症并引起Cx水平改变。尽管Pannexins（Panx）与Cx家族成员具有相似之处，但它们在糖尿病性肾病中的功能仍未完全确定。我们研究了PUFA补充对大鼠糖尿病肾脏中Px1和Cx家族成员免疫表达的影响。在实验DM1模型中的四组大鼠中补充不同的饮食n-6 / n-3比例；对照组（C）和糖尿病组（STZ）≈7，STZ + N6组≈60，STZ + N3组≈1（含16％EPA和19％DHA）。Cx40，Cx43，使用免疫组织化学方法评估了糖尿病大鼠肾脏组织中的Cx45和Panx1。糖尿病显着降低了肾皮质中Cx40和Cx43的蛋白表达，并提高了Panx1蛋白的表达（p <0.05-p <0.01）。饮食对Cx和Panx1免疫表达有显着影响。日粮中高n-6 / n-3比值的添加下调了糖尿病大鼠中Cx45和Panx1的蛋白表达（p <0.05-p <0.01），而高低n-6喂养的糖尿病大鼠中Cx43免疫表达增加。 / n-3比率（p <0.01-p <0.001）。DN中的高血糖状况会干扰细胞间通讯，并会由于连接蛋白和pannexin免疫表达的变化而干扰细胞及其周围环境之间的连接。