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ZmEHD1 Is Required for Kernel Development and Vegetative Growth through Regulating Auxin Homeostasis.
Plant Physiology ( IF 6.5 ) Pub Date : 2019-12-19 , DOI: 10.1104/pp.19.01336 Yafei Wang 1, 2 , Wenwen Liu 2 , Hongqiu Wang 1, 2 , Qingguo Du 2 , Zhiyuan Fu 1 , Wen-Xue Li 3 , Jihua Tang 1
Plant Physiology ( IF 6.5 ) Pub Date : 2019-12-19 , DOI: 10.1104/pp.19.01336 Yafei Wang 1, 2 , Wenwen Liu 2 , Hongqiu Wang 1, 2 , Qingguo Du 2 , Zhiyuan Fu 1 , Wen-Xue Li 3 , Jihua Tang 1
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The roles of C-terminal Eps15 homology domain (EHD) proteins in clathrin-mediated endocytosis in plants are poorly understood. Here, we isolated a maize (Zea mays) mutant, designated ehd1, which showed defects in kernel development and vegetative growth. Positional cloning and transgenic analysis revealed that ehd1 encodes an EHD protein. Internalization of the endocytic tracer FM4-64 was substantially reduced in the ehd1 mutant and ZmEHD1 knockout mutants. We further demonstrated that ZmEHD1 and the ZmAP2 σ subunit physically interact at the plasma membrane. Auxin distribution and ZmPIN1a-YFP localization were altered in the ehd1 mutant. Kernel indole-3-acetic acid levels were substantially lower in the ehd1 mutant than in wild-type maize. Exogenous application of 1-naphthaleneacetic acid, but not GA3 or 2-naphthaleneacetic acid, rescued the seed germination and seedling emergency phenotypic defects of ehd1 mutants. Taken together, these results indicate that ZmEHD1 regulates auxin homeostasis by mediating clathrin-mediated endocytosis through its interaction with the ZmAP2 σ subunit, which is crucial for kernel development and vegetative growth of maize.
中文翻译：
ZmEHD1是通过调节生长素体内稳态来促进内核发育和营养生长所需的。
人们对C端Eps15同源域（EHD）蛋白在网格蛋白介导的内吞作用中的作用了解甚少。在这里，我们分离了一个玉米突变体，命名为ehd1，该突变体显示出籽粒发育和营养生长方面的缺陷。位置克隆和转基因分析表明ehd1编码EHD蛋白。内吞示踪剂FM4-64的内在化在ehd1突变体和ZmEHD1敲除突变体中大大减少。我们进一步证明ZmEHD1和ZmAP2σ亚基在质膜上发生物理相互作用。生长素的分布和ZmPIN1a-YFP本地化在ehd1突变体中发生了变化。ehd1突变体中的吲哚-3-乙酸水平明显低于野生型玉米。外用1-萘乙酸，但不施用GA3或2-萘乙酸，挽救了ehd1突变体的种子发芽和幼苗紧急表型缺陷。综上所述，这些结果表明ZmEHD1通过介导网格蛋白介导的内吞作用，通过与ZmAP2σ亚基的相互作用来调节植物生长素稳态，这对玉米的籽粒发育和营养生长至关重要。
更新日期：2020-03-03
中文翻译：
ZmEHD1是通过调节生长素体内稳态来促进内核发育和营养生长所需的。
人们对C端Eps15同源域（EHD）蛋白在网格蛋白介导的内吞作用中的作用了解甚少。在这里，我们分离了一个玉米突变体，命名为ehd1，该突变体显示出籽粒发育和营养生长方面的缺陷。位置克隆和转基因分析表明ehd1编码EHD蛋白。内吞示踪剂FM4-64的内在化在ehd1突变体和ZmEHD1敲除突变体中大大减少。我们进一步证明ZmEHD1和ZmAP2σ亚基在质膜上发生物理相互作用。生长素的分布和ZmPIN1a-YFP本地化在ehd1突变体中发生了变化。ehd1突变体中的吲哚-3-乙酸水平明显低于野生型玉米。外用1-萘乙酸，但不施用GA3或2-萘乙酸，挽救了ehd1突变体的种子发芽和幼苗紧急表型缺陷。综上所述，这些结果表明ZmEHD1通过介导网格蛋白介导的内吞作用，通过与ZmAP2σ亚基的相互作用来调节植物生长素稳态，这对玉米的籽粒发育和营养生长至关重要。
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