This study examined the effects of a carbohydrate-restricted diet on aging, brain function, intestinal bacteria and the life span to determine long-term carbohydrate-restriction effects on the aging process in senescence-accelerated prone mice (SAMP8). Three-week-old male SAMP8 were divided into three groups after a week of preliminary feeding. One group was given a controlled diet, while the others fed on high-fat and carbohydrate-restricted diets, respectively. The mice in each group were further divided into two subgroups, of which one was the longevity measurement group. The other groups fed ad libitum until the mice were 50 weeks old. Before the test period termination, passive avoidance test evaluated the learning and memory abilities. Following the test period, serum and various mice organs were obtained and submitted for analysis. The carbohydrate-restricted diet group exhibited significant decrease in the survival rate as compared to the other two diet groups. The passive avoidance test revealed a remarkable decrease in the learning and memory ability of carbohydrate-restricted diet group as compared to the control-diet group. Measurement of lipid peroxide level in tissues displayed a marked increase in the brain and spleen of carbohydrate-restricted diet group than the control-diet and high-fat diet groups. Furthermore, notable serum IL-6 and IL-1β level (inflammation indicators) elevations, decrease in Enterobacteria (with anti-inflammatory action), increase in inflammation-inducing Enterobacteria and lowering of short-chain fatty acids levels in cecum were observed in the carbohydrate-restricted diet group. Hence, carbohydrate-restricted diet was revealed to promote aging and shortening of life in SAMP8.

这项研究检查了限制碳水化合物饮食对衰老，易衰老小鼠（SAMP8）衰老过程的长期限制作用，以确定其对衰老，脑功能，肠道细菌和寿命的影响。在一周的初步喂养后，将三周大的雄性SAMP8分为三组。一组给予控制饮食，而另一组分别以高脂饮食和限制碳水化合物饮食为食。每组中的小鼠进一步分为两个亚组，其中一个是寿命测量组。其他人群自由进食直到小鼠50周大。在测试期终止之前，被动回避测试评估了学习和记忆能力。在测试期之后，获得血清和各种小鼠器官并进行分析。与其他两个饮食组相比，限制碳水化合物饮食组的存活率显着降低。被动回避测试显示与限制饮食组相比，限制碳水化合物饮食组的学习和记忆能力显着下降。对组织中脂质过氧化物水平的测量显示，与限制饮食和高脂饮食组相比，限制碳水化合物饮食组的大脑和脾脏显着增加。此外，血清IL-6和IL-1β水平（炎症指标）显着升高，血清IL-6和IL-1β降低。在限制碳水化合物的饮食组中观察到肠杆菌（具有抗炎作用），引起炎症的肠杆菌的增加以及盲肠中短链脂肪酸水平的降低。因此，发现限制碳水化合物的饮食可促进SAMP8的衰老并缩短其寿命。