Green tea polyphenols (GTPs) exhibit beneficial effects towards obesity and intestinal inflammation; however, the mechanisms and association with gut microbiota are unclear. We examined the role of the gut microbiota of GTPs treatment for obesity and inflammation. Canines were fed either a normal diet or high-fat diet with low (0.48% g/kg), medium (0.96% g/kg), or high (1.92% g/kg), doses of GTPs for 18 weeks. GTPs decreased the relative abundance of Bacteroidetes and Fusobacteria and increased the relative abundance of Firmicutes as revealed by 16S rRNA gene sequencing analysis. The relative proportion of Acidaminococcus, Anaerobiospirillum, Anaerovibrio, Bacteroides, Blautia, Catenibactetium, Citrobacter, Clostridium, Collinsella, and Escherichia were significantly associated with GTPs-induced weight loss. GTPs significantly (P<.01) decreased expression levels of inflammatory cytokines, including TNF-α, IL-6, and IL-1β, and inhibited induction of the TLR4 signaling pathway compared with high-fat diet. We show that the therapeutic effects of GTPs correspond with changes in gut microbiota and intestinal inflammation, which may be related to the anti-inflammatory and anti-obesity mechanisms of GTPs.

绿茶多酚（GTP）对肥胖和肠道炎症表现出有益的作用。然而，尚不清楚肠道菌群的机制和相关性。我们检查了GTPs肠道菌群对肥胖和炎症的治疗作用。用低剂量（0.48％g / kg），中剂量（0.96％g / kg）或高剂量（1.92％g / kg）的普通饮食或高脂饮食喂养犬18周。通过16S rRNA基因测序分析，GTP降低了拟杆菌和梭菌的相对丰度，并增加了Firmicutes的相对丰度。的相对比例氨基酸球菌，厌氧螺，Anaerovibrio，类杆菌，Blautia，Catenibactetium，柠檬酸杆菌属，梭菌属，柯林斯，大肠杆菌和大肠埃希菌与GTPs引起的体重减轻显着相关。绿茶多酚显著（P <0.01）减少炎性细胞因子，包括TNF-α，IL-6，和IL-1β，和TLR4信号传导途径的抑制诱导具有高相比的表达水平-脂肪饮食。我们表明，GTP的治疗效果与肠道菌群和肠道炎症的变化相对应，这可能与GTP的抗炎和抗肥胖机制有关。