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Tweaking genome-editing approaches for virus interference in crop plants.
Plant Physiology and Biochemistry ( IF 6.1 ) Pub Date : 2019-12-19 , DOI: 10.1016/j.plaphy.2019.12.022 Muntazir Mushtaq 1 , Shazia Mukhtar 1 , Aafreen Sakina 2 , Aejaz Ahmad Dar 1 , Rohini Bhat 3 , Rupesh Deshmukh 4 , Kutubuddin Molla 5 , Ajaz Ahmad Kundoo 6 , Mohd Saleem Dar 7
Plant Physiology and Biochemistry ( IF 6.1 ) Pub Date : 2019-12-19 , DOI: 10.1016/j.plaphy.2019.12.022 Muntazir Mushtaq 1 , Shazia Mukhtar 1 , Aafreen Sakina 2 , Aejaz Ahmad Dar 1 , Rohini Bhat 3 , Rupesh Deshmukh 4 , Kutubuddin Molla 5 , Ajaz Ahmad Kundoo 6 , Mohd Saleem Dar 7
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Plant viruses infect various economically important crops and cause a serious threat to agriculture. As of now, conventional strategies employed are inadequate to circumvent the proliferation of rapidly evolving plant viruses. In this regard, recent advancement in genome-editing approach looks promising to produce plants resistant to DNA/RNA virus infections. Clustered regularly interspaced palindromic repeats (CRISPR) system has been emerged as a promising genome-editing tool that has received special interest because of its ease, competence and reproducibility. Recent studies have demonstrated that CRISPR/Cas9 system has great potential to confer plant immunity by either directly targeting or cleaving the viral genome in both RNA and DNA viruses. Similarly, the approach can be used for targeting the host susceptibility genes more particularly in case of RNA viruses. In the present review, different approaches and strategies being used to improve plant resistance against devastating viruses are discussed in view of recent advances in CRISPR systems. This review also describes the major pitfalls of CRISPR/Cas9 system that utilizes highly efficient and novel platforms to engineer interference to single and multiple plant RNA viruses.
中文翻译：
调整基因组编辑方法以抑制作物中的病毒。
植物病毒感染各种重要的经济作物，并对农业造成严重威胁。到目前为止，所采用的常规策略不足以规避迅速发展的植物病毒的繁殖。在这方面，基因组编辑方法的最新进展有望产生对DNA / RNA病毒感染具有抗性的植物。簇状规则的间隔回文重复（CRISPR）系统已经成为一种有前途的基因组编辑工具，由于其易用性，能力和可重复性而受到了特别的关注。最近的研究表明，通过直接靶向或切割RNA和DNA病毒中的病毒基因组，CRISPR / Cas9系统具有赋予植物免疫力的巨大潜力。相似地，该方法可用于靶向宿主易感基因，尤其是在RNA病毒的情况下。在本综述中，鉴于CRISPR系统的最新进展，讨论了用于提高植物对毁灭性病毒的抗性的不同方法和策略。这篇综述还描述了CRISPR / Cas9系统的主要陷阱，该系统利用高效和新颖的平台来设计对单个和多个植物RNA病毒的干扰。
更新日期：2019-12-20
中文翻译：
调整基因组编辑方法以抑制作物中的病毒。
植物病毒感染各种重要的经济作物，并对农业造成严重威胁。到目前为止，所采用的常规策略不足以规避迅速发展的植物病毒的繁殖。在这方面，基因组编辑方法的最新进展有望产生对DNA / RNA病毒感染具有抗性的植物。簇状规则的间隔回文重复（CRISPR）系统已经成为一种有前途的基因组编辑工具，由于其易用性，能力和可重复性而受到了特别的关注。最近的研究表明，通过直接靶向或切割RNA和DNA病毒中的病毒基因组，CRISPR / Cas9系统具有赋予植物免疫力的巨大潜力。相似地，该方法可用于靶向宿主易感基因，尤其是在RNA病毒的情况下。在本综述中，鉴于CRISPR系统的最新进展，讨论了用于提高植物对毁灭性病毒的抗性的不同方法和策略。这篇综述还描述了CRISPR / Cas9系统的主要陷阱，该系统利用高效和新颖的平台来设计对单个和多个植物RNA病毒的干扰。
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