Many neurological disorders are associated with abnormal oscillatory dynamics. The suprachiasmatic nucleus (SCN) is responsible for the timing and synchronization of physiological processes. We performed experiments on PERIOD2::LUCIFERASE transgenic "knock-in" mice. In these mice, a gene that is expressed in a circadian pattern is fused to an inserted gene that codes for luciferase, which is a bioluminescent enzyme. A one-time 3 min magnetic stimulation (MS) was applied to excised slices of the SCN. The MS consisted of a 50-mT field that was turned on and off 4,500 times. The rise time and fall time of the field were 75 μs. A photon count that extended over the full 5 days that the slice remained viable, subsequently revealed how the MS affected the circadian cycle. The MS was applied at points in the circadian cycle that correspond to either maximal or minimal bioluminescence. It was found that both the amplitude and period of the endogenous circadian oscillation are affected by MS and that the effects strongly depend on where in the circadian cycle the stimulation was applied. Our MS dose is in the same range as clinically applied doses, and our findings imply that transcranial MS may be instrumental in remedying disorders that originate in circadian rhythm abnormalities. Bioelectromagnetics. 2020;41:63-72 © 2019 Wiley Periodicals, Inc.

中文翻译：

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在具有磁刺激的体外超视交叉核中扰动昼夜节律振荡。

许多神经系统疾病与异常的振荡动力学有关。视交叉上核（SCN）负责生理过程的时间安排和同步。我们对PERIOD2 :: LUCIFERASE转基因“敲入”小鼠进行了实验。在这些小鼠中，以昼夜节律模式表达的基因与编码荧光素酶的插入基因融合，荧光素酶是一种生物发光酶。将一次3分钟的磁刺激（MS）应用于SCN的切片。MS由一个50 mT的磁场组成，该磁场被打开和关闭4,500次。磁场的上升时间和下降时间为75μs。光子计数持续了整整5天，使切片保持活力，随后揭示了MS如何影响昼夜节律周期。在昼夜周期中对应于最大或最小生物发光的点处应用MS。已经发现，内源性昼夜节律振荡的幅度和周期均受MS影响，并且其影响在很大程度上取决于在昼夜节律周期中施加刺激的位置。我们的MS剂量与临床应用剂量处于同一范围内，我们的发现暗示经颅MS可能有助于补救由昼夜节律异常引起的疾病。生物电磁学。2020; 41：63-72©2019 Wiley Periodicals，Inc. 我们的MS剂量与临床应用剂量处于同一范围内，我们的发现暗示经颅MS可能有助于补救由昼夜节律异常引起的疾病。生物电磁学。2020; 41：63-72©2019 Wiley Periodicals，Inc. 我们的MS剂量与临床应用剂量处于同一范围内，我们的发现暗示经颅MS可能有助于补救由昼夜节律异常引起的疾病。生物电磁学。2020; 41：63-72©2019 Wiley Periodicals，Inc.