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Dynamic hyperpolarized 13 C MR spectroscopic imaging using SPICE in mouse kidney at 9.4 T.
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2019-12-19 , DOI: 10.1002/nbm.4230
Jae Eun Song 1 , Jaewook Shin 1 , Hansol Lee 1 , Young-Suk Choi 2 , Ho-Taek Song 2 , Dong-Hyun Kim 1
Affiliation  

This study aims to investigate the feasibility of dynamic hyperpolarized 13 C MR spectroscopic imaging (MRSI) using the SPectroscopic Imaging by exploiting spatiospectral CorrElation (SPICE) technique and an estimation of the spatially resolved conversion constant rate (kpl ). An acquisition scheme comprising a single training dataset and several imaging datasets was proposed considering hyperpolarized 13 C circumstances. The feasibility and advantage of the scheme were investigated in two parts: (a) consistency of spectral basis over time and (b) accuracy of the estimated kpl . The simulations and in vivo experiments support accurate kpl estimation with consistent spectral bases. The proposed method was implemented in an enzyme phantom and via in vivo experiments. In the enzyme phantom experiments, spatially resolved homogeneous kpl maps were observed. In the in vivo experiments, normal diet (ND) mice and high-fat diet (HFD) mice had kpl (s-1 ) values of medullar (ND: 0.0119 ± 0.0022, HFD: 0.0195 ± 0.0005) and cortical (ND: 0.0148 ±0.0023, HFD: 0.0224 ±0.0054) regions which were higher than vascular (ND: 0.0087 ±0.0013, HFD: 0.0132 ±0.0050) regions. In particular, the kpl value in the medullar region exhibited a significant difference between the two diet groups. In summary, the feasibility of using modified SPICE for dynamic hyperpolarized 13 C MRSI was demonstrated via simulations and in vivo experiments. The consistency of spectral bases over time and the accuracy of the estimated kpl values validate the proposed acquisition scheme, which comprises only a single training dataset. The proposed method improved the spatial resolution of dynamic hyperpolarized 13 C MRSI, which could be used for kpl estimation using high signal-to-noise ratio spectral bases.

中文翻译:

使用SPICE在9.4 T小鼠肾脏中进行动态超极化13 C MR光谱成像。

这项研究旨在通过利用空间光谱相关性(SPICE)技术和空间分辨的转换恒定速率(kpl)的估计,来研究使用分光镜成像技术进行动态超极化13 C MR光谱成像(MRSI)的可行性。考虑到超极化的13 C环境,提出了一个包含单个训练数据集和几个成像数据集的采集方案。该方案的可行性和优势分两部分进行了研究:(a)频谱基础随时间推移的一致性以及(b)估计kpl的准确性。仿真和体内实验支持使用一致的光谱库进行准确的kpl估计。所提出的方法是在酶模体中并通过体内实验实现的。在酵素体模实验中 观察到空间分辨的均质kpl图。在体内实验中,正常饮食(ND)小鼠和高脂饮食(HFD)小鼠的髓质(ND:0.0119±0.0022,HFD:0.0195±0.0005)和皮层(ND:0.0148)的kpl(s-1)值±0.0023,HFD:0.0224±0.0054)区域高于血管(ND:0.0087±0.0013,HFD:0.0132±0.0050)区域。特别是,在两个饮食组之间,延髓区域的kpl值显示出显着差异。总之,通过模拟和体内实验证明了将修饰的SPICE用于动态超极化13 C MRSI的可行性。频谱库随时间的一致性以及估计的kpl值的准确性验证了建议的采集方案,该方案仅包含单个训练数据集。
更新日期:2020-02-04
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