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Spectral Imaging for Microbubble Characterization.
Langmuir ( IF 3.7 ) Pub Date : 2019-12-19 , DOI: 10.1021/acs.langmuir.9b03828 Richard J Browning 1 , Miles Aron 1 , Anna Booth 1, 2 , Paul Rademeyer 1 , Sarah Wing 1 , Veerle Brans 1 , Shamit Shrivastava 1 , Dario Carugo 1, 3 , Eleanor Stride 1
Langmuir ( IF 3.7 ) Pub Date : 2019-12-19 , DOI: 10.1021/acs.langmuir.9b03828 Richard J Browning 1 , Miles Aron 1 , Anna Booth 1, 2 , Paul Rademeyer 1 , Sarah Wing 1 , Veerle Brans 1 , Shamit Shrivastava 1 , Dario Carugo 1, 3 , Eleanor Stride 1
Affiliation
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Microbubbles stabilized by an outer lipid shell have been studied extensively for both diagnostic and therapeutic applications. The shell composition can significantly influence microbubble behavior, but performing quantitative measurements of shell properties is challenging. The aim of this study is to investigate the use of spectral imaging to characterize the surface properties of a range of microbubble formulations representing both commercial and research agents. A lipophilic dye, C-laurdan, whose fluorescence emission varies according to the properties of the local environment, was used to compare the degree and uniformity of the lipid order in the microbubble shell, and these measurements were compared with the acoustic response and stability of the different formulations. Spectral imaging was found to be suitable for performing rapid and hence relatively high throughput measurements of microbubble surface properties. Interestingly, despite significant differences in lipid molecule size and charge, all of the different formulations exhibited highly ordered lipid shells. Measurements of liposomes with the same composition and the debris generated by destroying lipid microbubbles with ultrasound (US) showed that these exhibited a lower and more varied lipid order than intact microbubbles. This suggests that the high lipid order of microbubbles is due primarily to compression of the shell as a result of surface tension and is only minimally affected by composition. This also explains the similarity in acoustic response observed between the formulations, because microbubble dynamics are determined by the diameter and shell viscoelastic properties that are themselves a function of the lipid order. Within each population, there was considerable variability in the lipid order and response between individual microbubbles, suggesting the need for improved manufacturing techniques. In addition, the difference in the lipid order between the shell and lipid debris may be important for therapeutic applications in which shedding of the shell material is exploited, for example, drug delivery.
中文翻译：
光谱成像用于微泡表征。
由脂质外层壳稳定的微泡已被广泛研究用于诊断和治疗应用。壳的成分可以显着影响微泡行为，但是对壳的性能进行定量测量是具有挑战性的。这项研究的目的是研究使用光谱成像来表征一系列代表商业和研究人员的微泡制剂的表面特性。亲脂性染料C-laurdan的荧光发射随当地环境的特性而变化，用于比较微泡壳中脂质顺序的程度和均匀性，并将这些测量值与声学响应和稳定性进行比较。不同的配方。发现光谱成像适合于进行微泡表面特性的快速且因此相对较高的通量测量。有趣的是，尽管脂质分子的大小和电荷存在显着差异，但所有不同的制剂均表现出高度有序的脂质壳。对具有相同组成的脂质体以及通过超声（美国）破坏脂质微泡而产生的碎片的测量显示，与完整的微泡相比，脂质体的脂质顺序更低且变化更大。这表明微泡的高脂质级主要是由于表面张力导致壳的压缩所致，并且仅受组成的影响最小。这也解释了配方之间观察到的声学响应的相似性，因为微泡动力学取决于直径和壳粘弹性，它们本身是脂质顺序的函数。在每个人群中，各个微泡之间的脂质顺序和反应存在很大差异，这表明需要改进制造技术。另外，壳与脂质碎片之间的脂质顺序差异对于其中利用壳材料脱落（例如药物递送）的治疗应用可能是重要的。
更新日期：2020-01-10
中文翻译：
光谱成像用于微泡表征。
由脂质外层壳稳定的微泡已被广泛研究用于诊断和治疗应用。壳的成分可以显着影响微泡行为，但是对壳的性能进行定量测量是具有挑战性的。这项研究的目的是研究使用光谱成像来表征一系列代表商业和研究人员的微泡制剂的表面特性。亲脂性染料C-laurdan的荧光发射随当地环境的特性而变化，用于比较微泡壳中脂质顺序的程度和均匀性，并将这些测量值与声学响应和稳定性进行比较。不同的配方。发现光谱成像适合于进行微泡表面特性的快速且因此相对较高的通量测量。有趣的是，尽管脂质分子的大小和电荷存在显着差异，但所有不同的制剂均表现出高度有序的脂质壳。对具有相同组成的脂质体以及通过超声（美国）破坏脂质微泡而产生的碎片的测量显示，与完整的微泡相比，脂质体的脂质顺序更低且变化更大。这表明微泡的高脂质级主要是由于表面张力导致壳的压缩所致，并且仅受组成的影响最小。这也解释了配方之间观察到的声学响应的相似性，因为微泡动力学取决于直径和壳粘弹性，它们本身是脂质顺序的函数。在每个人群中，各个微泡之间的脂质顺序和反应存在很大差异，这表明需要改进制造技术。另外，壳与脂质碎片之间的脂质顺序差异对于其中利用壳材料脱落（例如药物递送）的治疗应用可能是重要的。
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