HIV latency can be established in vitro following direct infection of a resting CD4+ T cell (pre‐activation latency) or infection of an activated CD4+ T cell which then returns to a resting state (post‐activation latency). We modified a previously published dual‐fluorescent reporter virus seeking to track the establishment and reactivation of pre‐activation latency in primary CD4+ T cells.

中文翻译：

---

双荧光 HIV 报告病毒在预激活潜伏期模型中的局限性

HIV潜伏期可以在直接感染静息CD4+ T细胞（激活前潜伏期）或感染激活的CD4+ T细胞后返回静息状态（激活后潜伏期）后在体外建立。我们修改了之前发表的双荧光报告病毒，试图追踪原代 CD4+ T 细胞中预激活潜伏期的建立和重新激活。