当前位置：
X-MOL 学术
›
Cancer Med.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Autoantibodies against tumor-associated antigens combined with microRNAs in detecting esophageal squamous cell carcinoma.
Cancer Medicine ( IF 2.9 ) Pub Date : 2019-12-19 , DOI: 10.1002/cam4.2792 Guiying Sun 1, 2 , Hua Ye 1, 2 , Xiao Wang 3 , Tiandong Li 1, 2 , Di Jiang 2, 3, 4 , Cuipeng Qiu 1, 2 , Liping Dai 2, 3 , Jianxiang Shi 2, 3 , Kaijuan Wang 1, 2 , Chunhua Song 1, 2 , Peng Wang 1, 2 , Jianying Zhang 1, 2, 3
Cancer Medicine ( IF 2.9 ) Pub Date : 2019-12-19 , DOI: 10.1002/cam4.2792 Guiying Sun 1, 2 , Hua Ye 1, 2 , Xiao Wang 3 , Tiandong Li 1, 2 , Di Jiang 2, 3, 4 , Cuipeng Qiu 1, 2 , Liping Dai 2, 3 , Jianxiang Shi 2, 3 , Kaijuan Wang 1, 2 , Chunhua Song 1, 2 , Peng Wang 1, 2 , Jianying Zhang 1, 2, 3
Affiliation
|
Esophageal carcinoma (EC) is a common malignant disease worldwide, especially in China. There is currently no specific blood test for detecting EC. Autoantibodies against tumor-associated antigens (TAAbs) and microRNAs (miRNAs) are promising markers for cancer diagnosis and this study focuses on combining TAAbs and miRNAs to evaluate the diagnostic value in esophageal squamous cell carcinoma (ESCC). The expression levels of seven TAAbs and five microRNAs in plasmas from 125 patients diagnosed with ESCC and 125 healthy individuals were detected by enzyme-linked immunosorbent assay (ELISA) and reverse transcription quantitative-polymerase chain reaction (RT-qPCR), respectively. The receiver operating characteristic (ROC) analysis was applied to estimate the diagnostic value of these markers for distinguishing ESCC patients from normal individuals. Logistic regression analysis was performed to generate prediction model and calculate the probability of individuals being diagnosed with ESCC. Three panels were established including four TAAbs, three miRNAs, and three TAAbs combined with three miRNAs. The panel consisting of three TAAbs (HCCR, C-myc, and MDM2) and three miRNAs (miR-21, miR-223, and miR-375) attained great diagnostic value for ESCC, with an area under the receiver operating characteristic curve (AUC) of 0.89 (95% CI: 0.85-0.93) with the sensitivity of 69%, the specificity of 90%, the PPV of 83%, the NPV of 79%, and the coincidence rate of 81%. The optimal panel of six-member markers was able to effectively discriminate the patients with ESCC from normal individuals, especially for early esophageal squamous cell carcinoma.
中文翻译：
抗肿瘤相关抗原的自身抗体与 microRNA 联合检测食管鳞状细胞癌。
食管癌（EC）是世界范围内尤其是我国的常见恶性肿瘤。目前尚无用于检测 EC 的特定血液测试。针对肿瘤相关抗原 (TAAb) 和 microRNA (miRNA) 的自身抗体是有前景的癌症诊断标志物，本研究重点是将 TAAb 和 miRNA 结合起来评估食管鳞状细胞癌 (ESCC) 的诊断价值。采用酶联免疫吸附试验（ELISA）和逆转录定量聚合酶链反应（RT-qPCR）分别检测125名食管鳞癌患者和125名健康个体血浆中7种TAAb和5种microRNA的表达水平。应用受试者工作特征（ROC）分析来估计这些标志物对于区分 ESCC 患者与正常个体的诊断价值。进行逻辑回归分析以生成预测模型并计算个体被诊断患有食管癌的概率。建立了三个组，包括四个 TAAb、三个 miRNA 和三个 TAAb 与三个 miRNA 组合。由三种 TAAb（HCCR、C-myc 和 MDM2）和三种 miRNA（miR-21、miR-223 和 miR-375）组成的组合对 ESCC 具有巨大的诊断价值，其受试者工作特征曲线下面积（ AUC）为0.89（95% CI：0.85-0.93），敏感性为69%，特异性为90%，PPV为83%，NPV为79%，符合率为81%。最佳的六人标记物组能够有效区分食管鳞癌患者与正常个体，尤其是早期食管鳞状细胞癌。
更新日期：2019-12-19
中文翻译：
抗肿瘤相关抗原的自身抗体与 microRNA 联合检测食管鳞状细胞癌。
食管癌（EC）是世界范围内尤其是我国的常见恶性肿瘤。目前尚无用于检测 EC 的特定血液测试。针对肿瘤相关抗原 (TAAb) 和 microRNA (miRNA) 的自身抗体是有前景的癌症诊断标志物，本研究重点是将 TAAb 和 miRNA 结合起来评估食管鳞状细胞癌 (ESCC) 的诊断价值。采用酶联免疫吸附试验（ELISA）和逆转录定量聚合酶链反应（RT-qPCR）分别检测125名食管鳞癌患者和125名健康个体血浆中7种TAAb和5种microRNA的表达水平。应用受试者工作特征（ROC）分析来估计这些标志物对于区分 ESCC 患者与正常个体的诊断价值。进行逻辑回归分析以生成预测模型并计算个体被诊断患有食管癌的概率。建立了三个组，包括四个 TAAb、三个 miRNA 和三个 TAAb 与三个 miRNA 组合。由三种 TAAb（HCCR、C-myc 和 MDM2）和三种 miRNA（miR-21、miR-223 和 miR-375）组成的组合对 ESCC 具有巨大的诊断价值，其受试者工作特征曲线下面积（ AUC）为0.89（95% CI：0.85-0.93），敏感性为69%，特异性为90%，PPV为83%，NPV为79%，符合率为81%。最佳的六人标记物组能够有效区分食管鳞癌患者与正常个体，尤其是早期食管鳞状细胞癌。
京公网安备 11010802027423号