Abstract Wound dressings based on nanofiber polymer scaffolds with good antimicrobial performance and skin reconstruction ability are promising options to thwart wound infection and accelerate wound healing. This paper reports on the synthesis via electrospinning of chitosan-alginate (CS-Alg) nanofiber dressings with various amounts of gentamicin (Gn; 0–10 wt%) as a drug delivery system. Smooth and continuous nanofibers with no obvious beads were created, with increases in the amount of Gn resulting in reduced fiber diameter. Antimicrobial tests showed the Gn-loaded nanofibers had good antibacterial performance as indicated by the inhibition of bacterial growth. CS-Alg nanofibers loaded with higher Gn concentrations exhibited greater antibacterial performance than those with lower Gn concentrations. In vitro cell culture studies demonstrated that CS-Alg wound dressings with 1–3% Gn improved L929 cell attachment and proliferation more than wound dressings with higher Gn concentrations. In vivo experiments revealed that Cs-Alg nanofibers loaded with 3% Gn significantly enhanced skin regeneration in a Balb/C mice model by stimulating the formation of a thicker dermis, increasing collagen deposition, and increasing the formation of new blood vessels and hair follicles. Collectively, Gn-loaded CS-Alg wound dressings can be considered a good candidate for drug delivery systems and skin regeneration applications.

中文翻译：

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具有高抗菌性能的壳聚糖-海藻酸盐/庆大霉素纳米纤维伤口敷料的体内外评价

摘要 基于纳米纤维聚合物支架的伤口敷料具有良好的抗菌性能和皮肤重建能力，是阻止伤口感染和加速伤口愈合的有希望的选择。本文报道了通过静电纺丝合成壳聚糖-藻酸盐 (CS-Alg) 纳米纤维敷料和不同量的庆大霉素 (Gn; 0-10 wt%) 作为药物递送系统。产生了没有明显珠子的光滑和连续的纳米纤维，随着 Gn 量的增加导致纤维直径减小。抗菌试验表明，载有 Gn 的纳米纤维具有良好的抗菌性能，可抑制细菌生长。负载较高 Gn 浓度的 CS-Alg 纳米纤维比那些具有较低 Gn 浓度的纳米纤维表现出更好的抗菌性能。体外细胞培养研究表明，含有 1-3% Gn 的 CS-Alg 伤口敷料比具有较高 Gn 浓度的伤口敷料更能改善 L929 细胞的附着和增殖。体内实验表明，负载 3% Gn 的 Cs-Alg 纳米纤维通过刺激更厚的真皮的形成、增加胶原蛋白沉积以及增加新血管和毛囊的形成，显着增强了 Balb/C 小鼠模型的皮肤再生。总的来说，载有 Gn 的 CS-Alg 伤口敷料可以被认为是药物输送系统和皮肤再生应用的良好候选者。体内实验表明，负载 3% Gn 的 Cs-Alg 纳米纤维通过刺激更厚的真皮的形成、增加胶原蛋白沉积以及增加新血管和毛囊的形成，显着增强了 Balb/C 小鼠模型的皮肤再生。总的来说，载有 Gn 的 CS-Alg 伤口敷料可以被认为是药物输送系统和皮肤再生应用的良好候选者。体内实验表明，负载 3% Gn 的 Cs-Alg 纳米纤维通过刺激更厚的真皮的形成、增加胶原蛋白沉积以及增加新血管和毛囊的形成，显着增强了 Balb/C 小鼠模型的皮肤再生。总的来说，载有 Gn 的 CS-Alg 伤口敷料可以被认为是药物输送系统和皮肤再生应用的良好候选者。