Self-renewal is required for embryo stem cells (ESCs) and adipose-derived mesenchymal stem cells (ADMSCs). This study examined the ability of ferulic acid in mouse ESCs and ADMSCs, in a high fat diet-induced mouse model. Initially, five natural compounds of ferulic acid, xanthohumol, curcumin, ascorbic acid, and quercetin were screened in ESCs using an alkaline phosphate ⁺(AP⁺) assay, as a self-renewal biomarker. A ferulic acid treatment was the highest AP⁺ staining in hop-hit screening compounds. Also a ferulic acid increased Nanog mRNA levels in ESCs. The in vivo effects of ferulic acid were next examined in an obese mouse model. C57BL/6 J male mice were fed either a high fat diet (HFD) or control diet with ferulic acid (5 g/kg diet) for 8 weeks. Ferulic acid exhibited weight loss and improved glucose homeostasis, lipid profiling, and hepatic steatosis in a HFD-induced mouse model. Next, ADMSCs (Sca-1⁺CD45⁻), a hallmark of fat stem cells, were then isolated and quantified from mouse abdominal adipose tissue. A HFD decreased the Sca-1⁺CD45⁻ cell population of ADMSCs, but HFD-induced obese mice given ferulic acid showed an increased the Sca-1⁺CD45⁻ cell population of ADMSCs. Moreover, ferulic acid enhanced NANOG mRNA levels in human ADMSCs and its related gene mRNA expression. Overall, this study suggests that ferulic acid preserves self-renewal in ESCs, and contributes to ADMSCs self-renewal and effective weight control in obesity.

胚胎干细胞（ESC）和脂肪来源的间充质干细胞（ADMSC）需要自我更新。这项研究检查了高脂饮食诱导的小鼠模型中小鼠ESC和ADMSC中阿魏酸的能力。最初，使用碱性磷酸盐⁺（AP ⁺）测定法作为自更新生物标记，在ESC中筛选了阿魏酸，黄腐酚，姜黄素，抗坏血酸和槲皮素的5种天然化合物。阿魏酸治疗是最高的AP ⁺跳跃筛选化合物的染色。阿魏酸还增加了ESC中的Nanog mRNA水平。接下来在肥胖小鼠模型中检查阿魏酸的体内作用。给C57BL / 6 J雄性小鼠喂饲高脂饮食（HFD）或对照饮食并加入阿魏酸（5 g / kg饮食）8周。在HFD诱导的小鼠模型中，阿魏酸具有减肥作用，并改善了葡萄糖稳态，脂质分布和肝脂肪变性。接着，的ADMSCs（SCA-1 ⁺ CD45 ^- ），脂肪的干细胞的标志，然后分离并从小鼠腹部脂肪组织定量。甲HFD的的Sca-1降低⁺ CD45 ^-的ADMSCs的细胞群，但HFD诱导的肥胖小鼠给予阿魏酸显示出增加了的Sca-1 ⁺ CD45⁻ ADMSC的细胞群。此外，阿魏酸增强人ADMSCs中的NANOG mRNA水平及其相关基因的mRNA表达。总体而言，这项研究表明，阿魏酸可以在ESC中保持自我更新，并有助于肥胖中ADMSC的自我更新和有效体重控制。