The increase in soil salinization due to global climate change could cause large losses in crop productivity affecting, among other biological processes, to germination and seedling development. We have studied how salt stress affects nucleic acid degrading activities in radicles of common bean during seedling development. In radicles of common bean, a main nuclease of 37 kDa and two ribonucleases of 17 and 19 kDa were detected. Saline stress did not alter these three activities but induced a new ribonuclease of 16 kDa. All three ribonucleases are acidic enzymes that were inhibited by Zn. The 16 and 17 kDa ribonucleases are inhibited by guanilates. In the genome of common bean, we have identified 13 genes belonging to the T2 ribonuclease family and that are grouped in the 3 classes of T2 ribonucleases. The analysis of the expression of the 3 genes belonging to Class I (PvRNS1 to 3) and the unique gene from Class II (PvRNS4) in radicles showed that PvRNS3 is highly induced under salt stress.

中文翻译：

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响应盐胁迫的核酸酶和核糖核酸酶活性：鉴定PvRNS3，一种受盐胁迫诱导的普通豆胚中的T2 / S样核糖核酸酶。

由于全球气候变化而导致的土壤盐碱化的增加可能导致作物生产力的重大损失，这不仅影响其他生物过程，还影响发芽和幼苗发育。我们已经研究了盐胁迫如何影响幼苗发育过程中普通小豆的胚根中的核酸降解活性。在普通豆的胚根中，检测到一个37 kDa的主核酸酶和两个17 kDa和19 kDa的核糖核酸酶。盐胁迫并没有改变这三个活性，而是诱导了一个新的16 kDa的核糖核酸酶。所有三个核糖核酸酶都是被Zn抑制的酸性酶。16 kDa和17 kDa的核糖核酸酶被鸟苷酸抑制。在普通豆的基因组中，我们已经鉴定出13个属于T2核糖核酸酶家族的基因，这些基因被归类为T2核糖核酸酶的3类。