Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.,The Plant Cell

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Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
The Plant Cell ( IF 10.0 ) Pub Date : 2019-12-18 , DOI: 10.1105/tpc.19.00714
Yukihiro Nagashima ₁ , Zeyang Ma ₂ , Xueting Liu ₃ , Xiaoning Qian ₃ , Xiuren Zhang _{2,

4} , Antje von Schaewen ₅ , Hisashi Koiwa _{4,

6}

Affiliation

Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance. After insertion into the endoplasmic reticulum, KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM). From the TGN, the protein is targeted to growing cell plates during cell division. These processes are governed by multiple sequence motifs and also host genotypes. Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway. Conventional steady-state localization showed that structurally compromised KOR1 variants were directed to tonoplasts. In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway. Observations suggest the presence of multiple checkpoints/branches during KOR1 trafficking, where the destination is determined based on KOR1's sequence motifs and folding status. Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.

中文翻译：

ER 和 TGN 中的多种质量控制机制决定 KORRIGAN1 的亚细胞动力学和盐胁迫耐受功能。

在植物分泌系统内的众多糖蛋白中，KORRIGAN1 (KOR1) 是一种参与纤维素生物合成的膜锚定内切 β-1,4-葡聚糖酶，它提供了 N-糖基化、细胞壁生物合成和非生物胁迫耐受性之间的联系。插入内质网后，KOR1 在跨高尔基体网络 (TGN) 和质膜 (PM) 之间循环。来自 TGN 的蛋白质在细胞分裂期间靶向生长的细胞板。这些过程由多个序列基序和宿主基因型控制。在这里，我们研究了 KOR1 中已知和新识别的分选信号的相互作用和层次结构，以及它们如何影响分泌途径各个阶段的 KOR1 转运。传统的稳态定位表明，结构受损的 KOR1 变体针对液泡膜。此外，串联荧光计时器技术允许对年轻与老化的 KOR1 蛋白进行差异可视化，从而能够分析通过分泌途径的单程运输。观察结果表明，KOR1 运输过程中存在多个检查点/分支，其中目的地是根据 KOR1 的序列基序和折叠状态确定的。此外，对主要 PM 限制的 KOR1-L48L49→A48A49 变体的生长分析揭示了盐胁迫期间从 PM 中主动去除 KOR1 的重要性，否则会干扰胁迫驯化。

更新日期：2020-02-06

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